논문 상세보기
pp.515-524
본 연구에서는 RNAi 기작을 이용하여 식미에 중요한 영향을 미치는 아밀로스 함량을 다양화하기 위해 GBSSI 유전자의 3’-UTR 부위를 targeting하여 dsRNA를 생성시킬 수 있는 운반체를 제작하고, 벼에 형질전환 하였다. 작성된 형질전환체들을 대상으로 I2-KI 용액 반응과 아밀로스 함량을 분석한 결과, I2-KI 용액에 대한 반응은 waxy 타입으로 나타났으나 아밀로스 함량은 찰벼와 저아밀로스 벼 사이에 해당되는 범위를 보였다. 원품종과
The amylose content of starch is a major factor in the texture of cooked cereal grains. Therefore, down-regulation of amylose synthesis is one of the alternative method to improve eating quality of rice. We developed transgenic rice plants designed to suppress granule-bound starch synthase I(GBSSI) gene using RNA interference(RNAi) technology. Transgenic plants with RNAi vector containing the 3’-UTR region of GBSSI showed a lower amylose content in rice endosperm than that of wild-type. The range of amylose content was 5.9~9.0% in the transgenic plants, whereas that of wild-type was 17.7~18.0%. Transgenic rices showed the decrease of short chain and the increase of long chain by analyzing chain length distribution of amylopectin in the endosperm. In the SEM micrographs, we found that compound starch granules in whole grains of the wild-type rice were readily split during fracturing, while the starch granules in RNAi-transgenic lines showed small voluminous, non-angular rounded bodies.
