This study was performed to compared the effectiveness of individual treatments (electrolyzed water: EW, organic acid, and ultrasound) and their combination on reducing foodborne pathogens from perilla leaves. Perilla leaves were innoculated with a cocktail of Salmonella Typhimurium, Staphylococcus aureus, Bacillus cereus. Inoculated perilla leaves were treated with EW combined with different concentration of acetic acid (0.5%, 1.0%, 1.5%, 2.0%) for 1 min at room temperature. Treatment of 3 pathogens on perilla leaves with electrolyzed water combined with ultrasound (25 kHz) and 0.5% acetic acid was also performed for 1 min. While the numbers of S. Typhimurium and B. cereus showed reduced with increasing acetic acid concentration, there is no difference in the number of S. aureus treated with EW containing 0.5% to 1.5% acetic acid. Discoloration was observed the perilla leaves treated with EW combined with more than 1.0% acetic acid. For all three pathogens, the combined treatment of EW and ultrasound resulted in additional 0.42 to 0.72 log10 CFU/g. The maxium reductions of S. Typhimurium and B. cereus were 0.95, 1.23 log10 CFU/g after treatment with EW combined with 0.5% acetic acid and ultrasound simultaneously. The results suggest that the treatment of EW combined with 0.5% acetic acid and ultrasound increased pathogens reduction compared to individual treatment.

ABSTRACT
 재료 및 방법
  사용된 균주
  전해수 준비
  병용처리 유기산 선정
  Acetic acid 병용처리에 따른 들깻잎 중 미생물 저감화 효과분석
  초음파와 acetic acid 병용처리에 의한 미생물 저감화 상승효과
  미생물 분석
  통계처리
 결과 및 고찰
  유기산 첨가에 따른 전해수의 미생물 저감효과
  들깻잎에서 acetic acid 병용처리에 따른 미생물 저감화효과
  초음파와 acetic acid 병용처리에 따른 전해수의 미생물저감화
 요 약
 감사의 글
 참고문헌

• 김세리(농촌진흥청 국립농업과학원 농산물안전성부, Department of Agro-Food Safety, NAAS, RDA) | Se-Ri Kim
• 오기원(농촌진흥청 연구정책국, Research Policy Bureau, RDA) | Ki-Won Oh
• 이명희(농촌진흥청 국립식량과학원 기능성작물부, Department of Functional Crop, NICS, RDA) | Myoung-Hee Lee
• 정찬식(농촌진흥청 국립식량과학원 기능성작물부, Department of Functional Crop, NICS, RDA) | Chan-Sik Jung
• 이서현(농촌진흥청 국립농업과학원 농산물안전성부, Department of Agro-Food Safety, NAAS, RDA) | Seo-Hyun Lee
• 박선자(경남과학기술대학교 제약공학과, Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) | Sun-Ja Park
• 박정현(계명대학교 전통미생물자원개발 및 산업화연구센터, The Center for Traditional Microorganism Resources, Keimyung University) | Jung-Hyun Park
• 류경열(농촌진흥청 연구정책국, Research Policy Bureau, RDA) | Kyoung Yul Ryu
• 김병석(농촌진흥청 국립농업과학원 농산물안전성부, Department of Agro-Food Safety, NAAS, RDA) | Byung-Seok Kim
• 김두호(농촌진흥청 국립농업과학원 농산물안전성부, Department of Agro-Food Safety, NAAS, RDA) | Doo-Ho Kim
• 윤종철(농촌진흥청 국립농업과학원 농산물안전성부, Department of Agro-Food Safety, NAAS, RDA) | Jong-Chul Yun
• 정덕화(경상대학교 응용생명과학부, Division of Applied Life Science, Gyeongsang National University) | Duck-Hwa Chung Correspondence to