본 연구에서는 참죽나무 잎 추출물이 에탄올로 유도된 산화스트레스로부터 간세포를 보호하며, 항산화 효소 유전자 발현을 증가시킴으로써 항산화 활성을 보이는 것으로 사료된다. 또한, 참죽나무 잎에는 플라보노이드가 다량 함유되어 있으며, 특히 퀘르세틴 배당체인 qercetin 3-O-rhamnoside(quercitrin)는 건조중량 100 g당 1,456.0±12.5 mg으로 총 플라보놀 함량(1,874.5±15.5 mg)의 77.7%를 차지하여 참죽나무잎의 주요 플라보놀 성분으로 확인되었다. 결론적으로 참죽 나무 잎 추출물은 알코올에 의한 간 세포의 손상을 보호하고, 산화스트레스를 감소시켰으며, 이는 참죽나무 잎의 주요 플라보노이드인 퀘르세틴과 비교했을 때도 그 효과가 우수함을 확인하였다. 따라서 참죽나무 잎은 알코올에 의해 손상된 간을 보호하는 식품으로서 이용가치가 높을 것으로 기대된다.

All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.

Abstract
 서 론
 재료 및 방법
  1. 참죽나무 주정 추출물 제조
  2. 참죽나무 잎의 플라보노이드(flavonoid) 추출 및 분석
  3. 세포 배양
  4. 세포보호 효과
  5. ROS 생성 억제능
  6. RT-PCR
  7. 통계처리
 결 과
  1. 참죽나무 잎의 플라보노이드 조성 및 함량
  2. 참죽나무 잎 추출물의 에탄올에 따른 HepG2 세포 독성 보호 효과
  3. 참죽나무잎추출물의활성산소종(ROS) 생성억제효과
  4. 참죽나무 잎 추출물의 항산화 효소 mRNA 발현 증가효과
 고 찰
 요약 및 결론
 References

• 김현정(농촌진흥청 국립농업과학원) | Hyun-Jeong Kim (National Institute of Agricultural Science, Rural Development Administration (RDA))
• 조수연(농촌진흥청 국립농업과학원) | Su-Yeon Cho (National Institute of Agricultural Science, Rural Development Administration (RDA))
• 김정봉(농촌진흥청 국립농업과학원) | Jung-Bong Kim (National Institute of Agricultural Science, Rural Development Administration (RDA))
• 김헌웅(농촌진흥청 국립농업과학원) | Heon-Woong Kim (National Institute of Agricultural Science, Rural Development Administration (RDA))
• 최정숙(농촌진흥청 국립농업과학원) | Jeong-Sook Choe (National Institute of Agricultural Science, Rural Development Administration (RDA))
• 장환희(농촌진흥청 국립농업과학원) | Hwan-Hee Jang (National Institute of Agricultural Science, Rural Development Administration (RDA)) Corresponding author