This study was conducted to investigate the effect of chronic alcohol supplementation on muscle atrophy in growing rats. Eighteen male Sprague Dawley rats were randomly divided into two groups: CG group (control group, n=9) and AG group (alcohol supplemented group, n=9). Alcohol group (3 g/kg BW) was orally supplemented every day. After the experimental period, serum components and muscle Akt, p-Akt, FoxO, p-FoxO, MuRF1, and P38 protein expressions were analyzed. In the results, the values of EDL and soleus muscle weights of AG group did not have significant differences compared to the value of the CG group. In the serum components, the value of the serum TG concentration of AG group was significantly increased compared to the value of the CG group. The value of the p-Akt/Akt and p-FoxO/FoxO of the AG group was significantly decreased compared to the value of the CG group (p<0.01). The MuRF1 protein expression of AG group was significantly increased compared to the value of the CG group (p<0.01). However, the values of p-P38/P38 between two groups did not have any significant difference. From these results, it was suggested that 4 weeks of chronic alcohol supplementation induced muscle atrophy via activated protein degradation pathway involving the inhibition of Akt phosphorylation and increased FoxO and MuRF1 protein expression of muscle in growing rats.

Abstract
 서 론
 재료 및 방법
  실험동물 및 알코올 섭취
  혈청 성분 분석
  근육조직 전처리
  Western Blotting
  자료처리 방법
 결과 및 고찰
  체중, 식이섭취량, 식이효율 및 조직 중량
  혈액 성분 변화
  Akt 및 p-Akt 단백질 발현
  FoxO, p-FoxO, MuRF1, p-P38 및 P38 단백질 발현
 감사의 글
 References

• 배상훈(선문대학교 체육학과 대학원, Department of Physical Education, Graduate School of Sunmoon University) | Sanghoon Bae
• 노성환(선문대학교 체육학과 대학원, Department of Physical Education, Graduate School of Sunmoon University) | Sunghwan No
• 박재용(선문대학교 스포츠건강과학연구소, Institute of Sports Health Science, Sunmoon University) | Jaeyong Park
• 송영주(선문대학교 스포츠건강과학연구소, Institute of Sports Health Science, Sunmoon University) | Youngju Song Corresponding author