A total of 16 growing pigs (Landrace × Yorkshire × Duroc, average weight 71 ± 2 kg) were used in this experiment for 24 days to determine the effect of replacing ground corn with barley varieties on growth performance, nutrient digestibility, and blood metabolites. Each pig was housed individually in a metabolic cage with a nipple waterer and feeder. Four dietary treatments included control (a corn and soybean meal-based diet) and 3 barley varieties (Youngyang, Wooho, or Yuyeon barley replacing 40% of ground corn). Growth performance and nutrient digestibility were not affected by replacement of 40% of ground corn with one of the three barley varieties (p > 0.05), but final body weight was. As for blood characteristics, pig treated with all of the barley treatments had no effect (p > 0.05) on total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LD), and glucose in comparison with control; however, there was a difference in high density lipoprotein (HDL)-cholesterol levels between the control and all of the barley replaced treatment groups (p < 0.05). In conclusion, in spite of statistically significant differences, each of the three barley varieties is a suitable substitute for ground corn in the diet of growing and fattening pigs without adverse effects on growth performance, nutrient digestibility, and blood characteristics.