논문 상세보기
pp.1307-1314
This study was conducted to isolate and characterize a novel feather-degrading bacterium producing keratinase activity. A strain K9 was isolated from soil at poultry farm and identified as Xanthomonas sp. K9 by phenotypic characters and 16S rRNA gene analysis. The cultural conditions for the keratinase production were 0.3% fructose, 0.1% gelatin, 0.04% K2HPO4, 0.06% KH2PO4, 0.05% NaCl and 0.01% FeSO4 with an initial pH 8.0 at 30℃ and 200 rpm. In an optimized medium containing 0.1% chicken feather, production yield of keratinase was approximately 8-fold higher than the yield in basal medium. The strain K9 effectively degraded chicken feather meal (67%) and duck feather (54%), whereas human nail and human hair showed relatively low degradation rates (13-22%). Total free amino acid concentration in the cell-free supernatant was about 25.799 mg/l. Feather hydrolysate produced by the strain K9 stimulated growth of red pepper, indicating Xanthomonas sp. K9 could be not only used to increase the nutritional value of chicken feather but also a potential candidate for the development of natural fertilizer applicable to crop plant soil.
1. 서 론
2. 재료 및 방법
2.1. 우모 분해세균의 분리 및 동정
2.2. Keratinase 생산 조건 검토
2.3. 다양한 케라틴 기질의 분해능 검토
2.4. Keratinase 활성 측정
2.5. 우모 분해산물의 조제 및 분석
2.6. 우모 분해산물의 농작물 생육촉진 효과 평가
3. 결과 및 고찰
3.1. 우모 분해세균의 분리 및 동정
3.2. Keratinase 생산 최적조건
3.3. 천연 케라틴 기질의 분해능
3.4. 우모 분해산물의 아미노산 함량
3.5. 우모 분해산물의 고추 생육촉진 효과
4. 결 론
참 고 문 헌
