논문 상세보기
pp.391-400
Background: Atractylodes radix is a well-known medicinal crop having many physiological effects. This study was conducted to select useful Atractylodes japonica × Atractylodes macrocephala (AJM) cultivars by comparing anti-oxidative and anti-inflammatory efficacies.
Methods and Results: Seven extracts from AJM cultivars were used to treat lipopolysacchride (LPS)-treated BV2 cells, and the effects on cell viability and inhibition on reactive oxygen species (ROS) and nitric oxide (NO) production were analyzed. In vitro scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxynitrite (NOO−) radicals were also investigated. Contents of total phenol, atractylenolide I, and atractylenolide III in the AJM extracts were measured using high performance liquid chromatography (HPLC) or spectrophotometry. The experiments show that none of the seven extracts was cytotoxic above 89.2% at 20 - 250㎍/㎖. Extracts of Gowon, Dawon, Sangchul, and Huchul inhibited ROS generation in a dose-dependent manner, and Sangchul extract showed the highest inhibition on ROS production. All the AJM extracts showed effective inhibitory activity after on NO release in the LPS-treated BV2 cells, and Sangchul extract showed the highest activity. Sangchul extract had the most potent scavenging activities for NOO− and had some DPPH radical scavenging effect. Sangchul extract also had the highest content at total phenol and atractylenolide I content. Atractylenolide III was not detected in the AJM extracts.
Conclusions: The results suggested that Sangchul was the most useful anti-oxidative and anti-inflammatory resource among the AJM cultivars.
서 언
재료 및 방법
1. 실험재료
2. 시약
3. 세포주 및 배양방법
4. 세포증식률 측정
5. ROS 생성 억제능 분석
6. NO 생성 저해능 분석
7. DPPH 라디칼 소거능 분석
8. Peroxynitrite 소거능 분석
9. 총페놀 함량 측정
10. 주요 지표성분 분석
11. 통계분석
결과 및 고찰
1. 삽주 품종별 추출물의 수율
2. 삽주 품종별 추출물의 세포증식율
3. 삽주 품종별 추출물의 활성산소 (ROS) 생성 저해능
4. 삽주 품종별 추출물의 NO 생성 저해능
5. 삽주 품종별 추출물의 DPPH 소거능
6. 삽주 품종별 추출물의 peroxynitrite (ONOO−)소거능
7. 삽주 품종별 추출물의 지표성분 정량 결과
REFERENCE
