더덕은 폴리페놀, 알칼로이드, 아미노산 및 기타 화합물로 구성되어 있고, tangshenosideⅠ과 lobetyolin의 함량은 더덕의 주요 화합물로 알려져 있다. 본 연구에서는 UPLC를 이용하여 주요 성분 tangshenosideⅠ과 lobetyolin을 동시 분석하였다. 최적 추출조건으로는 50% 에탄올을 이용하여 온도 60℃, 1시간 추출하였고, UPLC를 이용한 빠르고 간편한 분석조건을 확립하고, 정확성, 정밀성, 직선성, 특이성 등 분석법의 유효성을 검증하였다. 지역별로 더덕을 추출하여 UPLC로 주요 성분 함량을 분석한 결과, tangshenosideⅠ과 lobetyolin의 함량은 0.36~3.54 mg/g, 0.24~1.29 mg/g으로 나타났다. 지역별 생육환경에 따라 함량 차이가 나타났으며, 지역별 품질 관리를 위한 객관적이고 과학적인 근거를 제시하여 국내에서 재배되는 더덕의 품질 평가에 대한 하나의 기준이 될 것으로 기대된다.

Deoduk (Codonopsis lanceolata) has a complex chemical composition that includes polyphenols, saponins, amino acids, and other unidentified compounds. The contents of tangshenoside and lobetyolin are considered as standard of quality evaluation of Deoduk. In this study, an ultra-performance liquid chromatography (UPLC) method was developed for the quantitative determination of the two marker constituents, tangshenoside and lobetyolin. The methods for determining the standards of quality were validated by measuring their linearity, specificity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using UPLC. Reversed-phase UPLC analysis was conducted quantitatively to identify individual tangshenoside and lobetyolin in Deoduk extracted with 50% (v/v) aqueous ethanol. We used 21 samples to carry out quantitative analysis of tangshenoside and lobetyolin. Based on their dry weights, the levels of tangshenoside and lobetyolin were 0.36~3.54 mg/g, 0.24~1.29 mg/g, respectively. These results will be valuable as basic data for standardization of Korean Deoduk.

Abstract
 서 론
 재료 및 방법
  1. 실험재료 및 시약
  2. 시료 제조
  3. UPLC 분석
  4. 분석법 검증
  5. 통계처리
 결과 및 고찰
  1. 최적 추출조건 설정
  2. 분석 조건 설정
  3. 분석법 검증
  4. UPLC 분석
 요약 및 결론
 References

• 황병순(농촌진흥청 국립농업과학원 농식품자원부) | Byung Soon Hwang (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
• 김지영(농촌진흥청 국립농업과학원 농식품자원부) | Ji Yeong Kim (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
• 장미(농촌진흥청 국립농업과학원 농식품자원부) | Mi Jang (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
• 김기창(농촌진흥청 국립농업과학원 농식품자원부) | Gi-Chang Kim (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
• 박영희(농촌진흥청 국립농업과학원 농식품자원부) | Young-Hee Park (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
• 황인국(농촌진흥청 국립농업과학원 농식품자원부) | In Guk Hwang (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) Corresponding author