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치자(Gardenia jasminoides Ellis fructus) 종자 추출물의 항산화 활성 및 지질과산화 저해능 KCI 등재

Antioxidant Activities and Lipid Peroxidation Inhibition Ability of Gardenia jasminoides Ellis Fructus Seed Extracts

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한국환경과학회지 (Journal of Environmental Science International)
한국환경과학회 (The Korean Environmental Sciences Society)
초록

The purpose of this study was to investigate the bioactivity of extracts from the seeds of Gardenia jasminoides Ellis fructus (GJE) found in Namhae, Korea. Extraction was performed using three solvents, 70% methanol, Distilled Water (DW), and Ethyl Acetate (EA). We determined the total phenol and phytic acid contents of the extracts to evaluate their nitrogen oxide scavenging activity, antioxidant activity, reducing power, and lipid peroxidation inhibition ability. The phytic acid content of GJE was found to be 1.157 mg PAE (Phytic Acid Equivalent) /g DW. The yields of the three extraction processes were as follows: DW, 36.61%; 70% methanol, 30.10%; and EA, 20.40%. The physiological activities of the extract solvents increased significantly with increasing concentrations (0.2, 0.4, and 0.6 mg/mL) (p<0.05), but were lower than those of ascorbic acid, BHA, and trolox. Total phenol content was the highest in the 70% methanol extract, followed by DW and EA extracts. Further, nitrogen oxide scavenging activity and antioxidant activity were the highest for the 70% methanol extract followed by DW and EA extracts. Based on these results, the bioactivities of the 70% methanol and DW extracts of GJ seeds were excellent. These extracts can be used as natural antioxidants

목차
Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험 재료 및 시료의 추출
  2.2. Phytic acid와 총 페놀 함량 측정
  2.3. 생리활성 측정
  2.4. 통계 처리
 3. 결과 및 고찰
  3.1. Phytic acid와 총 페놀 함량 및 수율
  3.2. Nitric oxide radical 소거활성
  3.3. Nitrite 소거활성
  3.4. β-carotene bleaching을 이용한 항산화 활성
  3.5. 환원력
  3.6. 지질과산화 저해 활성
 4. 결 론
 REFERENCES
저자
  • 강동수(전남대학교 해양바이오식품학과) | Dong-Soo Kang (Department of Marine Bio Food Science, Chonnam National University)
  • 진동혁(부산대학교 식품공학과) | Dong-Hyeok Jin (Department of Food Science and Technology, Pusan National University)
  • 오다영(부산대학교 식품공학과) | Da-Young Oh (Department of Food Science and Technology, Pusan National University)
  • 김한수(부산대학교 식품공학과) | Han-Soo Kim (Department of Food Science and Technology, Pusan National University) Corresponding author