Microorganisms capable of degrading trichloroethylene(TCE) using phenol as a induction substrate were isolated from industrial effluents and soil. The strain MS-64K which had the highest biodegradability was identified as the genus Micrococcus. The optimal conditions of medium for the growth and biodegradation of trichloroethylene were observed as follows; the initial pH 7.0, trichloroethylene 1,000ppm as the carbon source, 0.2% (NH_4)_2SO_4 as the nitrogen source, respectively. Lag period and degradation time on optimal medium were shorter than those on isolation medium. Growth on the optimal medium was increased. Addition of 0.1% Triton X-100 increased the growth rate of Micrococcus sp. MS-64K, but degradation was equal to optimal medium. Trichloroethylene degradation by Micrococcus sp. MS-64K was shown to fit logarithmic model when the compound was added at initial concentration of 1,000ppm.