Zr-Ti alloy powders were successfully synthesized by magnesium thermal reduction of metal chlorides. The evaporated and mixed gasses of were injected to liquid magnesium and the chloride components were reduced by magnesium leading to the formation of . The released Zr and Ti atoms were then condensed to particle forms inside the mixture of liquid magnesium and magnesium chloride, which could be dissolved fully in post process by 1~5% HCl solution at room temperature. By the fraction-control of individually injected and gasses, the final compositions of produced alloy powders were changed in the ranges of Zr-0 wt.%~20 wt.%Ti and their purity and particle size were about 99.4% and the level of several micrometers, respectively.

과학기술의 발달과 경제수준 향상 및 운동량의 부족으로 인한 비만증의 발생율이 높아지는 반면 체중 감량에 대한 관심도 증가하고 있다. 특히 이런 관심은 젊은 여성들 사이에서 발생하는 경향이 있으며, 지나친 체중감량은 건강에도 해를 미치지만 체중감량에 대한 자료가 거의 전무한 실정이다. 본 연구는 다이어트가 영양상태에 미치는 영향을 알아보고자 하여 제1보고로써 다이어트 실태에 대해 조사한 것이다. 1. 조사 대상자들은 19세(27%), 20세(25.3%)의 연령층이 가장 두터웠으며, 체중은 50.32±0.91kg, 신장은 160.90±0.47cm이었고, BMI는 18.73±4.89로 나타나 평균적으로 여윈 상태임을 보였다. 2. 조사 대상자들의 한달 용돈은 10∼15만원이 가장 많았으며, 부모님의 체형은 정상이거나 마른 분이 절반을 넘었다. 한편 아버지만 뚱뚱하신 경우보다는 어머니만 뚱뚱하신 분이 더 많은 것으로 나타났다. 3. 조사 대상자들의 식태도는 하루 한, 두끼만 섭취하며, 불규칙적으로 섭취하는 사람이 많았고, 과반수가 자신의 체형을 과대인식하는 것으로 나타나 체형의 기준을 바르게 선정할 수 있는 영양교육이 필요함을 보였다. 4. 조사 대상자들의 실제 체중은 50.32±0.91kg이었으나 이상체중은 46.94±0.39kg으로써 이상체중이 유의적으로 낮았다(p=0.0114). 5. 조사 대상자의 37.54%가 1997년 한 해 중 다이어트를 실시한 경험이 있었으며, 처음 다이어트를 실시한 시기는 고등학교, 대학생 때, 고등학교와 대학 사이의 순으로 나타나 주로 고등학교 이후에 다이어트를 하려는 이유로는 자기 신체에 대한 기대 및 느낌 등의 주관적인 이유가 주된 원인이었으며, 과반수 이상의 여대생이 다이어트를 실시해 본 경험(79.3%)을 가지고 있었고, 다이어트 실시 빈도는 가끔 실시하는 경우(38.25%)가 가장 많았다. 또한 다이어트의 실시기간은 일주일 미만인 사람이 가장 많았으며, 평균 체중감소량은 1∼2kg(37.69%), 2∼4kg(29.44%)이었으며, 과반수 이상이(76.65%) 감량된 체중을 유지하지 못하는 것으로 나타났다. 6. 가장 선호하는 다이어트 방법은 하루 한끼 혹은 그 이상을 굶는 방법, 운동, 하루 3끼를 모두 섭취하되 열량을 줄이는 방법 등의 순으로 나타나 결국 섭취열량을 제한하거나 소비열량을 늘이는 방법을 사용하는 것으로 나타났다. 7. 다이어트에 대한 정보는 주로 신문, 잡지(30.86%), 가족, 친구, 친척(28.86%) 등의 대중매체나 주위의 사람들로부터 얻는 것으로 나타났다. 8. 영양상태 지각도가 보통 이상인 경우는 아버지, 어머니 두 분 다 뚱뚱하지 않은 경우일 때는 116명(42.65%)이며, 두 분 다 뚱뚱한 경우는 8명(2.95%)로 나타나 부모가 정상체중이거나 마른 경우 그 자녀의 영양자각도가 그렇지 않은 부모의 자녀에 비해 높은 것을 알 수 있었다(p=0.004). 9. 영양상태 자각도는 자신의 체형을 적당하다, 약간 뚱뚱하다고 대답한 group이 다른 group에 비해 높았다(p=0.001).

양파를 생채로 착즙하여 분리한 액즙을 Brix 70%까지 감압농축시킨 것과 이어서 잔사에 ethyl alcohol을 가하여 추출한 것을 농축시켜 같이 합하고, 갈변방지를 위하여 1% cysteine의 첨가와 유화제로서 2% PGDR을 첨가하여 양파 oleoresin을 제조하고 저장중 품질 안정성을 검토하였다. 양파로부터 oleoresin에 추출된 당은 저장온도 5℃, 25℃ 및 40℃에서 매우 안정하여 저장 60일후까지도 함량변화는 거의 일어나지 않았다. 양파 oleoresin 추출 직후 갈변도를 나타내는 흡광도가 0.38로 담갈색을 띄었는데 5℃에서는 저장 60일 후에도 변화가 거의 없었으나, 25℃ 및 40℃에서 저장한 경우는 60일후 흡광도가 1.53 및 3.32로 증가하였고, cysteine을 1% 첨가한 경우는 대조구에 비하여 갈색 억제효과가 상당히 나타나 40℃에서 60일간 저장하였을 때의 흡광도는 대조구의 절반 수준으로 나타났다. 유화 안정성은 5℃ 저장의 경우 20일, 40일 및 60일후에 분리되지 않는 emulsion 층의 부피가 각각 96.8%, 94.1% 및 90.6%로 매우 안정하였고, 25℃ 및 40℃에서 60일 저장후는 83.2% 및 75.4%로 유화상태가 다소 불안정하였다. 생채 양파로부터 추출한 oleoresin을 저장시키지 않고 바로 대두유에 1% 첨가한 경우와 0.02% BHA를 첨가하였을 때 A.I. 는 각각 1.39 및 1.72를 나타내어 0.02% BHA를 첨가한 대두유의 유도기간 연장효과에 대하여 80.8%에 해당하는 항산화 활성을 나타내었고, 5℃ 및 25℃에서 60일 동안 저장한 양파 oleoresin을 대두유에 각각 1% 첨가하였을 때의 A.I. 는 1.37 및 1.30을 나타내어 실온 또는 그 이하의 온도에서는 양파 oleoresin이 가지는 항산화 활성은 매우 안정하였으며, 40℃에서 저장 60일후의 양파 oleoresin의 A.I. 는 1.08로 나타나 고온에서 저장기일이 길어질수록 양파 oleoresin의 항산화활성은 다소 불안정하였다. Overall odor intensity의 지표로서 total pyruvate 함량변화는 5℃, 25℃ 및 40℃에서 저장 60일후 잔존율이 각각 89.9%, 79.7% 및 65.2%로 저온에서는 상당히 안정하였다. 양파 oleoresin의 저장중 pyruvate 감소에 대한 반응속도 상수는 저장온도 범위 5∼10℃에서 1.381∼4.735mmol/ℓ·hr, Q10 값은 1.537∼1.694, 그리고 활성화 에너지는 11.649㎉/g mole이었다.

The purpose of this study was to develop environmentally friendly black dye by comparing Ketapang and Gallnut, that have been traditionally used as a black dye. As a result of FT-IR and UV-Vis spectrophotometer analysis, Ketapang was characterized by condensed tannin, while Gallnut showed hydrolyzable tannin. Due to the dyeability characteristic analysis, the color fastness of the non-mordant dye was slightly lowered to grades 1-3. In the dry-cleaning fastness test, both non-mordant and iron mordant were excellent, in grade 4-5, and the daylight fastness was excellent grade 3-5 too. The L* values of Ketapang were 26.93 (pre-mordant) and 29.39 (post-mordant), which were higher than those of Gallnut, 41.90 (pre-mordant) and 43.02 (post-mordant), indicating that Ketapang was more easily colored as a black dye than Gallnut.

Green synthesis of gold nanoparticles(GNPs) considered more ecofriendly and cost effective than other chemical methods use of dangerous reagents and solvents, improved material and energy efficiency and enhanced design of non-toxic products. In this study, we developed a green synthesis method for using Caulis in Taeniam (BCT). BCT were characterized by UV-vis, Zetasizer, TEM, XRD, and FTIR. The antioxidant activity of BCT was determined by DPPH and ABTS radical-scavenging assays, and heme oxygenase-1 induction in RAW 264.7 macrophages. The resulting BCT appeared spherical with an average diameter of 67.171.39 nm The aAntioxidant activity was increased in a dependent manner. To conclude, the green synthesis of BCT-GNPs was successful, and it appers to be useful in the for future applications.

This study was performed to identify and quantify the asbestos fibers released from two types of asbestos-cement slate roofs. One is a plant roof installed in 1987 which contained 15% chrysotile, and the other is a residential roof installed before 1983 which contained 12% chrysotile. The concentrations of asbestos fibers in air surrounding asbestos-cement slate roofs and in the falling water harvested from the same roofs on rainy days ranged from 0.0012 to 0.0018 f/mL and from 1,764 f/L to 10,584 f/L, respectively. The concentration of inorganic fibers in the soil around asbestos-cement slate roofs was from 217 to 348 f/g. With the above results, the excess lifetime cancer risk (ELCR) for the risk assessment of the asbestos fibers released from asbestos-cement slate based on US EPA IRIS (Integrated risk information system) model is within 5.5E-06 ~ 6.5E-06 levels which indicates that the levels do not exceed “the acceptable risk(1.0E-05)” recommended by WHO. The asbestos concentration in air, drained rainfall and soil around the plant slate roof was higher than that around residential slate roof, but the excess lifetime cancer risk (ELCR) from residential slate was higher than that from plant slate. This s

본 연구는 향기요법의 중재변인별 메타분석을 실시하여 향후 향 기요법에 있어 더 효과적이며 과학적인 연구를 수행하기 위한 기초 자료를 제시하고자 수행되었다. 메타분석 선정기준에 의거하여 총 29편의 논문을 선정하였으며, CMA프로그램으로 효과크기를 산출하였다. 연구결과, 전체효과크기는 ES =0.678로 중간 이상의 효 과를 나타내었다. 중재변인별로 효과크기를 산출한 결과, 대상별 로는 일반인 집단(ES =0.751)이 효과적이었다. 따라서 향기요법 은 일반인과 환자에 따라 효과가 다르게 나타날 수 있으며, 향후에 는 일반인집단과 환자집단별로 적절한 중재방법을 제시하는 연구 가 필요할 것이라 판단된다. 적용방법별은 건식흡입법(ES=0.684) 이 확산흡입법(ES=0.643)보다 효과적이었지만 모두 중간이상의 효과크기를 나타냈기 때문에 어떤 방법을 적용하더라도 효과적이 라 할 수 있다. 따라서 연구설계 시 사용자 편의에 따라 선택이 가능 하다고 판단된다. 또한, 향기요법 실험기간별 효과크기는 21-30일 이 효과적이었으며(ES =0.905) 실험횟수별 효과크기는 11-15회 (ES=1.095), 실험 1회당 시간별 효과크기는 2분(ES=1.333)이 효 과적이었다. 이는 한 달이라는 기간 동안 주 3회에 1회당 2분이 향 기요법에 가장 효과적인 연구방법이라 판단된다. 향기요법 종속변 인별 효과크기는 심리(ES=0.768)가 효과적이었다. 이는 향기요 법이 심신 이완이 증진되어 심리적 효과와 불안과 우울이 감소된다 는 이론과 일치되는 결과로 볼 수 있다(Hansen et al., 2006). 따라 서 향기요법이 심리적인 문제 개선에 매우 유용한 요법이므로 극도 의 심리적 불안정을 가진 정신질환자(우울증, 공황장애, 피해망상 증, 불안장애 등)에게 적극적으로 활용하고 연구할 필요가 있다. 본 연구는향기요법의 각 중재변인별 효과크기를 산출하여 향기 요법의 효과를 다각적으로 분석하였고 향기요법에 대한 후속연구 의 토대를 마련하였다는 점에서 의미가 있다. 그러나 향기요법의 구체적이고 세밀한 중재변인을 분석하는데 한계가 있었다. 예를 들 어 향기요법의 재료는 한 가지를 사용하는 것이 아니라 3가지를 혼 합하여 사용하기 때문에 메타분석으로 효과크기를 산출하기 어려 웠다. 추후연구에서는 이 같은 한계를 극복할 수 있는 연구가 필요 하다.

Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, K2HPO4 0.2%, NaH2PO4ㆍ2H2O 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at 26℃-36℃ with a maximum at 32 ℃. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15 mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium. Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose.

To treat chromaticity contained in effluents of dyeing wastewater efficiently, potent dye-degrading microorganisms were isolated from influent water, aeration- tank sludge, recycle water and settling-tank sludge located in leather and dyeing treatment plant. Six potent strains were finally isolated and identified as Comamonas testosteroni, Methylobacteriaceae bacterium, Stenotrophomonas sp., Kluyveromyces fragilis, Ascomycetes sp. and Basidiomycetes sp. When Basidiomycetes sp. was inoculated into ME medium containing basal mixed-dyes, 93% of color was removed after 8 days incubation. In the same experiment, the 1:1 mixed culture of Basidiomycetes sp. and photosynthetic bacterium exhibited 88% of color removal; however, it showed better color removal for single-color dyes. The aeration-tank and settling-tank samples revealed higher color removal (95-96%) for black dyes. The settling-tank sample also revealed higher color removal on basal mixed-dyes, which resulted in 90% color removal after 6-h incubation. From the above results, it is expected to achieve a higher color removal using the mixed microorganisms that were isolated from aeration-tank and settling-tank samples.

Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration (IC50) against tyrosinase activity was 0.36×104 mg/ml in ethanol extract, 0.11×103 mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.

This study was performed to evaluate the asbestos exposure levels and to calculate excess lifetime cancer risk (ELCR) for the risk assessment of the asbestos fibers released from asbestos-cement slate roofing (ASR) building. Total number of ASR buildings was into 21,267 in Busan, and 82.03 percent of the buildings was residential houses, and 43.61 percent of the buildings was constructed in 1970s. For this study, ten buildings were selected randomly among the ASR buildings. The range of airborne asbestos concentration in the selected ten ASR buildings was from 0.0016 to 0.0067 f/mL, and the concentration around no-admitted ASR buildings was higher than that around admitted buildings. The ELCR based on US EPA IRIS (integrated risk information system) model is within 3.5E-05 ~ 1.5E-04 levels, and the ELCR of no-admitted ASR buildings was higher than 1.0E-04 (one person per million) level that is considered a more aggressive approach to mitigate risk. These results indicate that the cancer risk from ASR buildings is higher than other buildings, and systematic public management is required for control of no-admitted ASR buildings within near future.

The concentration of organic compounds was analyzed at each step of BAC process though BDOCtotal/rapid/slow. Further, bacteria communities and biomass concentrations measured FISH and ATP methods were analyzed. The bed volume (BV) of steady state is different from that of based on assessment of organic compounds removal. Bed volumes in DOC, BDOCrapid and BDOCtotal/slow removal at steady state were around 27,500 (185.8 day), 15,000 (101.4 day) and 32,000 (216.2 day), respectively. A biomass didn't change after the bed volume reached 22,500 (152.0 day) according to analyzing ATP concentration of bacteria. The concentration of ATP was 2.14 μg/g in BV 22,500 (152.0 day). The total bacterial number was 4.01±0.4×107 cells/g at the bed volume 1,150 (7.8 day) (the initial operation) and the number of bacteria was 9.27±0.2×109 at the bed volume 58,560 395.7 day) that increased more than 200 times. Bacterial uptrend was reduced and bacterial communities were stabilized since BV 18,720 (126.5 day). When BV were 1,150 (7.8 day), 8,916 (60.2 day), 18,720 (126.5 day), 31,005 (209.5 day), 49,632 (335.3 day), 58,560 (395.7 day), a proportion of total bacteria for the Eubacteria were 60.1%, 66.0%, 78.4%, 82.0%, 81.3% respectively. γ-Proteobacteria group was the most population throughout the entire range. The correlation coefficient (r2) between Eubacteria biomass and ATP concentration was 0.9448.

We investigated usefulness of chicken feather as bioadsorbent for removal of hexavalent chromium[Cr(Ⅵ)] and oil from aqueous solution. Chicken feather was chemically treated with DTPA, EDTA, NaOH and SDS, respectively. Among them, EDTA was the most effective in adsorbing Cr(Ⅵ). Cr(Ⅵ) uptake by chicken feather was increased with decreasing pH; the highest Cr(Ⅵ) uptake was observed at pH 2.0. By increasing Cr(Ⅵ) concentration, Cr(Ⅵ) uptake was increased, and maximum Cr(Ⅵ) uptake was 0.34 mmol/g. Cr(Ⅵ) adsorption by chicken feather was well described by Freundlich isotherm than Langmuir isotherm and Freundlich constant(1/n) was 0.476. As the concentration of chicken feather was increased, Cr (Ⅵ) removal efficiency was increased but Cr(Ⅵ) uptake was decreased. Most of Cr(Ⅵ) was adsorbed at early reaction stage(1 h) and adsorption equilibrium was established at 5 h. On the other hand, chicken feather adsorbed effectively oils including bunker-A and bunker-C. In conclusion, our results suggest that chicken feather waste could be used to remove heavy metal and oil; it is a potential candidate for biosorption material.

We isolated and characterized novel duck feather-degrading bacteria producing keratinase. Twelve strains were isolated from soil and faces at poultry farm, and decayed feathers. They were identified as Bacillus methylotrophicus, Pseudomonas geniculata, Pseudomonas hibiscicola, Exiquobacterium profundum, Bacillus pumilus, Bacillus amyloliquefaciens, Chryseobacterium indologenes, Bacillus thuringiensis, Thermomonas koreensis, respectively, by phenotypic characters and 16S rRNA gene analysis. Generally, the level of keratinase production was not proportional to feather degradation rate. The highest keratinolytic activity was observed in the culture inoculated with Chryseobacterium indologenes D27. Although all strains did not degrade human hair, strains tested effectively degraded chicken feather(53.8-91.4%), wool(40.4-93.0%) and human nail (51.0-82.9%). These results suggest that strains isolated could be not only used to improve the nutritional value of recalcitrant feather waste but also is a potential candidate for biotechnological processes of keratin hydrolysis.

The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of MgCl₂·6H₂O and MgSO₄·7H₂O in the medium. Supplementation of medium with 0.01% CaCl₂·2H₂O and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% MgCl₂·6H₂O, 0.01% MgSO₄·7H₂O, 0.01% CaCl₂·2H₂O and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.

The bacterial community structure in biological activated carbon (BAC) process in drinking water treatment plant was investigated by Fluorescent in situ Hybridization (FISH) with rRNA-targeted oligonucleotide probe. Samples were collected at different three points in BAC process every month for one year. They were hybridized with a probe specific for the alpha, beta, gamma subclass of the class Proteobacteria, Cytophaga- Flavobacteria group and Gram-positive high G+C content (HGC) group. Total numbers of bacteria in BAC process counted by 4',6-diamidino-2-phenylindole (DAPI) staining were 5.4×1010 (top), 4.0×1010 (middle) and 2.8×1010 cells/ml (bottom). The number of the culturable bacteria was from 1.0×107 to 3.6×107 cells/ml and the culturability was about 0.05%. The faction of bacteria detectable by FISH with the probe EUB338 was about 83% of DAPI counts. Gamma and alpha subclass of the class Proteobacteria were predominant in BAC process and their ratios were over 20% respectively. In top and middle, alpha, beta and gamma subclass of the class Proteobacteria competed with each other and their percentages was changed according to the season. In bottom, gamma subclass of the class Proteobacteria was predominant all through the year. It could be successfully observed the seasonal distribution of bacterial community in biological activated carbon process using FISH.

Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates. The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2ㆍ6H2O 0.01%, respectively. The optimal temperature and initial pH was 30℃ and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was 113.8 ㎍/ml and 504.9 ㎍/ml, respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was 47.2 ㎍/ml and 334.0 ㎍/ml, respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.