The extracts of AS contain in alloxanthin, halocynthiaxanthin, astaxanthin and 13 kinds of carotenoids. The aim of the study was to assess the anti-oxidant activity and cell viability of AS. The anti-oxidant activity was determined by using DPPH radical inhibition activity and superoxide dismutase (SOD)-like activity. The results of cell viability assay showed that the extracts from AS were cytotoxic at concentrations above 5.0 ㎎/㎖. This study was designed to examine inflammation induced by LPS, protection effect by UVB and the toxicity of Ascidian shell extract(ASE) as a functional cosmetic ingredient. Evaluation of embryo toxicity resulted in embryo coagulation and mortality when treated at 5.0, 10.0, 20.0 ㎎/㎖. At the lowest concentration of 1.0 ㎎/㎖, hatchability resulted in 100.0 % rate. The results of arrhythmia measurement in larvae showed similarity to the evaluation of embryo toxicity. This result demonstrated that toxicity is present at concentrations greater than 5.0 ㎎/㎖. The protective effect of ASE on LPS and UVB-induced in the zebrafish was investigated. Intracellular reactive oxygen species(ROS) generated by the exposure of zebrafish to LPS, UVB-radiation were significantly decreased after treatment with ASE at 0.1 ㎎/㎖. As a result, ASE similarly reduced UVB-induced ROS generation and cell death in live zebrafsih. Therefore, it is suggested that ASE has anti-Inflammatory effects and can possibly be used as a functional substance for skin protection in the future.

Abstract
 1. Introduction
 2. Experiment
  2.1. Experimental materials
  2.2. Cell Culture
  2.3. Maintenance of zebrafish
  2.4. WST-1 assay
  2.5. DPPH assay
  2.6. SOD assay
  2.7. Zebrafish embryo toxicity test
  2.8. Zebrafish Arrhythmia test
  2.9. Estimation of intracellular ROSgeneration and generation and celldeath in zebrafish embryos andimage analysis for UV-B protected
  2.10. Estimation of LPS-stimulatedintracellular ROS and cell death inzebrafish embryos and imageanalysis
 3. Results and Discussion
  3.1. WST-1 cell viability assays
  3.2. Evaluation free radical DPPH assays
  3.3. Evaluation free radical SOD assays
  3.4. Zebrafish embryo toxicity assay
  3.5. Zebrafish Arrhythmia assay
  3.6. Effects of ASE on UVB-inducedintracellular ROS generation and celldeath in zebrafish
  3.7. Effects of ASE on LPS-stimulatedintracellular ROS generation and celldeath in zebrafish
 4. Conclusions
 References

• Sin-Ho Park(Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
• Bo-Ae Kim(Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
• Jae-Chan Yang(Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics) Corresponding author