The extracts of AS contain in alloxanthin, halocynthiaxanthin, astaxanthin and 13 kinds of carotenoids. The aim of the study was to assess the anti-oxidant activity and cell viability of AS. The anti-oxidant activity was determined by using DPPH radical inhibition activity and superoxide dismutase (SOD)-like activity. The results of cell viability assay showed that the extracts from AS were cytotoxic at concentrations above 5.0 ㎎/㎖. This study was designed to examine inflammation induced by LPS, protection effect by UVB and the toxicity of Ascidian shell extract(ASE) as a functional cosmetic ingredient. Evaluation of embryo toxicity resulted in embryo coagulation and mortality when treated at 5.0, 10.0, 20.0 ㎎/㎖. At the lowest concentration of 1.0 ㎎/㎖, hatchability resulted in 100.0 % rate. The results of arrhythmia measurement in larvae showed similarity to the evaluation of embryo toxicity. This result demonstrated that toxicity is present at concentrations greater than 5.0 ㎎/㎖. The protective effect of ASE on LPS and UVB-induced in the zebrafish was investigated. Intracellular reactive oxygen species(ROS) generated by the exposure of zebrafish to LPS, UVB-radiation were significantly decreased after treatment with ASE at 0.1 ㎎/㎖. As a result, ASE similarly reduced UVB-induced ROS generation and cell death in live zebrafsih. Therefore, it is suggested that ASE has anti-Inflammatory effects and can possibly be used as a functional substance for skin protection in the future.

본 연구는 멍게 껍질을 100.0 % 에탄올 용매로 추출한 후 DMSO로 희석하여 색소안정성을 평가하였다. pH에 따른 흡광도와 색차계 값을 측정한 결과 pH 7.0에서 흡광도와 색도의 ±a값이 가장 안정하게 나타났다. pH 3.0에서 흡광도의 감소가 나타났고 색도의 ±a값이 감소하여 멍게 껍질 색소는 pH가 중성에 가까울수록 변색방지의 효과가 있을 것으로 판단된다. 항산화제 첨가시 색소의 흡광도가 증가하였으며 α-tocopherol과 Glutathione이 가장 우수한 효과를 나타내었다.