In the case of foot-and-mouth disease (FMD), there is a great deal of impact on the national economy due to the disposal of diseases, the cost of disease control such as vaccination, reduction of productivity, and restriction of international trade of livestock products. Therefore, appropriate diagnostic methods for sensitive, accurate and rapid identification of virus serotypes are continuously required in terms of early prevention of FMD. This study was conducted to confirm the feasibility of immuno-PCR diagnostic method for the more sensitive detection of Korean FMD virus (FMDV). We synthesized a partial FMD type A viral gene. Protein antigen, monoclonal and polyclonal antibodies of FMDV were cloned, expressed and purified and then magnetic particles were attached to polyclonal antibodies and and oligomers to monoclonal antibodies for the immnuno-PCR. We confirmed the antigen-antibody and oligomer reaction using ELISA, Western blot, and real-time PCR. These results show that Korean FMDV can be detected by using difference of Ct values between positive group and negative group using immuno-PCR.. The results of this study also suggest that this technique will be the basis of the diagnosis method to detect Korean FMDV more sensitively in the future.

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 재조합 FMDV VP1 A 서열 합성 및 항원 제작
  2. 항체 제작 및 확인
  3. 항원과 항체의 반응 실험
  4. Antibody conjugation
  5. Immuno-PCR
 Ⅲ. 결과
  1. 재조합 FMDV VP1 A 및 epitope의 클로닝
  2. 제작한 항체의 반응성 확인
  3. 항원과 항체의 반응 실험 결과
  4. Antibody conjugation
  5. Immuno-PCR
 Ⅳ. 고찰
 Ⅴ. 요약
 Ⅵ. 참고문헌

• 곽경록(강원대학교 동물생명과학대학) | Kyeongrok Kwak (College of Animal Life Sciences, Kangwon National University)
• 이정근(㈜다우진유전자연구소) | Jeong Keun Lee (Dowgene)
• 최소영(강원대학교 동물생명과학대학) | So-young Choi (College of Animal Life Sciences, Kangwon National University)
• 황춘홍(㈜다우진유전자연구소) | Choon Hong Hwang (Dowgene)
• 이성진(강원대학교 동물생명과학대학) | Sung-Jin Lee (College of Animal Life Sciences, Kangwon National University) Corresponding author