This study investigated the anti-inflammatory effects of processed (Beopje) curly dock (Rumex crispus L.) in LPS (lipopolysaccharide)-stimulated murine RAW 264.7 cells. The experimental group was classified into five groups : LPS no treatment, CD (curly dock), CD-B (CD processed through Beopje), LPS, LPS+CD-B (LPS+CD processed through Beopje) and LPS+CD (LPS+CD). Treatment of the Raw 264.7 cell lines using LPS led to a significant increase in NO production, pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), and inflammation related genes (COX-2 and iNOS). Investigation of the inhibitory effects of CD and processed CD on NO production and expression of iNOS and COX-2 was done in LPS-induced RAW 264.7 cells. There was significant inhibition of NO production by LPS+CD and LPS+CD-B in a dose-dependent manner (p<0.05). Particularly, LPS+CD-B exhibited reduced mRNA expression of iNOS and COX-2 and NO production as compared to LPS+CD in Raw 264.7 cell lines (p<0.05). These results may explain some known biological activities of curly dock including the anti-inflammatory effects. CD-B in particular exhibited the highest anti-inflammatory effects of inhibiting production of NO, through the regulation of inflammatory related genes and pro-inflammatory cytokines. These results of Beopje processing might help decrease the anti-biological effects and increase several active substances of curly dock

Abstract
서 론
재료 및 방법
    1. 실험 재료 준비
    2. 마우스 대식세포 RAW 264.7 세포주 배양
    3. 생존율 분석
    4. Nitric oxide(NO) 농도 측정
    5. Enzyme-Linked Immunosorbent Assay(ELISA)
    6. Real Time - Quantitative Polymerase Chain Reaction(RT-qPCR)
    7. 통계처리
결과 및 고찰
    1. 세포 생존율에 미치는 영향
    2. Raw 264.7 세포에서 NO 생성
    3. Raw 264.7 세포에서pro-inflammatory cytokine의 함량
    4. Raw 264.7 세포에서 iNOS, COX-2의 mRNA 발현
요약 및 결론
References

• 김승희(호서대학교 벤처대학원 융합과학기술학과, 호서대학교 보건산업연구소) | Seung-Hee Kim (Dept. of Convergence Science and Technology, Graduate School of Venture, Hoseo University, Institute of Health Industry, Hoseo University)
• 강순아(호서대학교 벤처대학원 융합과학기술학과, 호서대학교 보건산업연구소) | Soon Ah Kang (Dept. of Convergence Science and Technology, Graduate School of Venture, Hoseo University, Institute of Health Industry, Hoseo University) Corresponding author