본 연구에서는 메주에서 분리된 C. allociferrii JNO301 을 이용한 최적 홍삼 ginsenosides의 생물전환 조건을 확인한 결과, 1.0% (v/v)의 홍삼 추출물에 탄소원으로서 2.5%(w/ v)의 galactose, 질소원으로서 1.0% (w/v)의 soytone을 첨가 하여 30oC에서 145 rpm으로 8일간 배양을 최적 생물전환 조건으로 확립하였다. C. allociferrii JNO301에 의해 홍삼 추출물에 존재하는 Rb1, Rb2, Rc, Rf가 생물전환되어 F2, Rh1, Rh2, compound O, compound Mc1, compound K 등 의 minor ginsenosides의 함량이 증가되었다.

Red ginseng (Panax ginseng C.A. Meyer) contains many functional components such as saponins, proteins, vitamins, and minerals. Ginsenosides are major functional components in the saponins of red ginseng. Candida allociferrii JNO301, which was isolated from Korean traditional meju, had β-glucosidase activity and major ginsenoside to minor ginsenoside conversion. The optimum conditions of carbon and nitrogen sources were 2.5%(w/v) of galactose and 1.0%(w/v) of soytone, while the fermentation time for the bioconversion of ginsenosides was 8 days at 30oC for 145 rpm. Under these conditions, C. allociferrii JNO301 converted ginsenosides Rb1, Rb2, Rc, and Rf into minor ginsenosides such as ginsenosides F2, Rh1, Rh2, compound O, compound Mc1, and compound K.

Abstract
서 론
재료 및 방법
    실험재료 및 시약
    생물전환 균주와 배양조건
    Thin layer chromatography (TLC)
    High-performance liquid chromatography (HPLC)
    탄소원 및 질소원에 따른 ginsenosides의 생물전환
결과 및 고찰
    탄소원에 따른 ginsenosides의 생물전환
    질소원에 따른 ginsenosides의 생물전환
    발효기간
    C. allociferrii JNO301를 이용한 ginsenosides의 생물전환경로
요 약
References

• 이설희(가천대학교 식품생물공학과/한국식품연구원 헬스케어연구단) | Sulhee Lee (Department of Food Science and Biotechnology, Gachon University/Research Group of Healthcare, Korea Food Research Institute)
• 이용훈(가천대학교 식품생물공학과) | Yong-Hun Lee (Department of Food Science and Biotechnology, Gachon University)
• 박영서(가천대학교 식품생물공학과) | Young-Seo Park (Department of Food Science and Biotechnology, Gachon University) Corresponding author