Highbush blueberries (Vaccinium corymbosum), ‘Duke’, ‘Bluecrop’, and ‘Elliott’, were applied with ground cover mulch systems to evaluate bush growth and fruit characteristics. Soil temperature variation was lower in the woodchip mulching plot. Therefore, CEC, soil pH, phosphate, mineral ions were not significantly different among mulching materials. The number of main stem was higher in woodchip > sawdust > rice husk > plastic film mulching plot, respectively. Total shoot number was also higher in organic mulching plots than in plastic film plot. However, total shoot number was not significantly different among the organic mulching plots. Fruit weight was the highest in the woodchip mulching plot of all blueberry cultivars, however, soluble sugar content, organic acidity, fruit firmness were not significantly different in mulching materials. Yield was higher in organic mulching plots than in plastic film plot in all blueberry cultivars. Especially, yield per shrub of woodchip plots was the highest about 3.6 kg in ‘Duke’, 2.7 kg in ‘Bluecrop’, and 2.6 kg in ‘Elliott’. Furthermore, correlation of main stem number and yield was highly significant (r2 = 0.8413). Therefore, woodchip mulching is expected to be an eco-friendly method to increase the number of stems and the yield of blueberries.