논문 상세보기
pp.130-142
This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.
I. 서 론
II. 연구내용 및 방법
1. 실험재료
2. 시료 준비
3. 총 폴리페놀 및 플라보노이드 함량
4. DPPH radical 소거능 측정
5. ABTS radical 소거능 측정
6. FRAP assay
7. ORAC assay
8. Pancreatic lipase 저해활성 측정
9. TBA assay
10. 3T3-L1 및 HepG2 세포 배양 및 분화
11. MTT assay
12. Oil red O staining
13. Triglyceride assay
14. 통계처리
III. 결과 및 고찰
1. 몽골 및 미얀마 식물의 총 폴리페놀 및 플라보노이드 함량
2. 몽골 및 미얀마 식물의 항산화 활성
3. 몽골 및 미얀마 식물의 Pancreatic lipase 저해 활성
4. 몽골 및 미얀마 식물의 지질 과산화물 함량 억제 활성
5. 몽골 및 미얀마 식물의 세포 독성 평가
6. 몽골 및 미얀마 식물의 3T3-L1 세포에서 지질 축적 및 중성지방 함량 억제 효과
7. 몽골 및 미얀마 식물의 HepG2 세포에서 지질 축적 및 중성지방 함량 억제 효과
IV. 요약 및 결론
References
