This study was designed to evaluate the effect of administering dietary tomato powder (TP; 1, 5, and 10%) to mice with high-fat diet (HFD)-induced obesity for 12 weeks. The TP used in this study was prepared using unmarketable tomatoes. Male C57BL/6J mice (n=60) were randomly divided into five groups, namely, CON, mice fed a basal diet (10% fat); HFD, mice fed HFD (60% fat); HFD+TP1, mice fed HFD (60% fat) supplemented with 1% TP; HFD+TP5, mice fed HFD (60% fat) supplemented with 5% TP; HFD+TP10, mice fed HFD (60% fat) supplemented with 10% TP. The HFD+TP10 group showed lower final body weight (34.23 g) than the HFD group (39.41 g), along with decreased epididymal fat weight (p<0.05). In addition, the HFD+TP10 group showed significantly lower serum cholesterol and triglyceride contents (136.32 and 33.20 mg/dL, respectively) that the HFD group (175.68 and 59.52 mg/dL, respectively). Increased serum leptin and insulin levels (66.36 and 1.80 ng/mL, respectively) in mice with HFD-induced obesity could be rescued in mice fed HFD supplemented with 10% TP (35.94 and 1.23 ng/mL, respectively). Additionally, the epididymal fat content and hepatic steatosis area showed a dose-dependent decrease with increase in dietary TP supplementation. The anti-obesity effect of 10% TP was linked to reduced serum trimethylamine-N-oxide levels. These results suggested that 10% TP was effective at inhibiting the accumulation of fat in the serum and tissue, and ameliorating lipid metabolism disorders observed in HFD-fed mice. In addition, such utilization of unmarketable tomato to inhibit obesity-associated pathologies is expected to add value and increase profits in the functional food industry.

ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 실험재료
    2. 실험동물 및 처리구
    3. 채혈 및 조직 적출
    4. 혈액 생화학적 분석
    5. 혈중 지질 함량 분석
    6. 간조직 내 지질 함량 분석
    7. 분변 내 지질 함량 분석
    8. 간조직 내 병리학적 관찰 및 지방증 면적 분석
    9. 부고환 지방 내 병리학적 관찰 및 지방 세포 크기 측정
    10. 혈청 내 trimethylamine-N-Oxide(TMAO) 농도 측정
    11. 분변 내 미생물 균총
    12. 통계처리
Ⅲ. 결과 및 고찰
    1. 체중 변화, 식이 섭취량, 및 식이효율
    2. 장기 무게
    3. 혈액 내 glucose, blood urea nitrogen, leptin, insulin농도
    4. 혈액, 간, 및 분변 내 지질성분 함량
    5. 간조직 조직학적 분석 및 지방증 면적
    6. 부고환 지방 조직학적 분석 및 지방세포 크기
    7. 혈청 내 trimethylamine-N-Oxide (TMAO) 함량
    8. 분변 내 미생물 균총
Ⅳ. 요약
Ⅴ. 참고문헌

• 김혜진(강원대학교 동물응용과학과) | Hye-Jin Kim (Department of Applied Animal Science, Kangwon National University)
• 장애라(강원대학교 원예학과) | Aera Jang (Department of Applied Animal Science, Kangwon National University)
• 이희철(강원대학교 원예학과) | Hee Cheol Lee (Department of Horticulture, Kangwon National University)
• 백민우(강원대학교 원예학과) | Min Woo Baek (Department of Horticulture, Kangwon National University)
• 정천순(강원대학교 원예학과) | Cheon Soon Jeong (Department of Horticulture, Kangwon National University) Corresponding Author