In this study, the effects of single and binary heavy metals toxicity on the growth and phosphorus removal ability of Bacillus sp.. known as be a phosphorus-removing microorganism, were quantitatively evaluated. Cd, Cu, Zn, Pb, Ni were used as heavy metals. As a result of analysis of variance of the half of inhibition concentration and half of effective concentration for each single heavy metal treatment group, the inhibitory effect on the growth of Bacillus sp. was Ni < P b < Z n < Cu < C d. A nd the inhibitory effect on phosphorus removal by Bacillus s p. w as N i < Pb < Z n < Cu < C d. When analyzing the correlation between growth inhibition and phosphorus removal efficiency of a single heavy metal treatment group, a negative correlation was found (R2 = 0.815), and a positive correlation was found when the correlation between IC50 and EC50 was analyzed (R2 = 0.959). In all binary heavy metal treatment groups, the interaction was an antagonistic effect when evaluated using the additive toxicity index method. This paper is considered to be basic data on the toxic effects of heavy metals when phosphorus is removed using phosphorus removal microorganisms in wastewater.

Abstract
1. 서 론
2. 재료 및 방법
    2.1. 미생물 배양 조건
    2.2. 합성 폐수
    2.3. 생장 측정 및 인산 정량
    2.4. IC50 및 EC50 추정
    2.5. 중금속 간 상호작용 평가
    2.6. 통계분석
3. 결과 및 고찰
    3.1. 단일 중금속
    3.2. 2종 혼합 중금속
4. 결 론
REFERENCES

• 김덕원(충북대학교 환경생명화학과) | Deok-Won Kim (Department of Environmental and Biological Chemistry, Chungbuk National University)
• 박지수(두산전자 Field Quality Control 김천part) | Ji-Su Park (Field Quality Control Gimcheon part, Doosan Corporation Electro-Materials)
• 오은지(한국환경연구원 자연환경연구실) | Eun-Ji Oh (Water and Land Research Group/Division for Natural Environment, Korea Environment Institute)
• 유진(인천광역시 보건환경연구원 생활환경과) | Jin Yoo (Indoor Environment Division, Incheon Research Institude of Public Health and Environment)
• 김덕현(국립환경과학원 토양지하수과) | Deok-Hyeon Kim (National Institute of Environmental Research)
• 정근욱(충북대학교 환경생명화학과) | Keun-Yook Chung (Department of Environmental and Biological Chemistry, Chungbuk National University) Corresponding author