This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography–photodiode array. The results showed that the correlation coefficient of calibration curve (R2) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 μg/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91- 102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.

ABSTRACT
Materials and Methods
    실험재료 및 시약
    지표성분 dieckol 분석
    표준용액 및 시험용액의 조제
    지표성분 분석법 유효성 검증
    특이성
    직선성
    정밀성 및 정확성
    검출한계 및 정량한계
Results and Discussion
    지표성분 dieckol의 크로마토그램을 이용한 특이성 확인
    검량선을 이용한 직선성 확인
    정밀성 및 정확성
    검출한계 및 정량한계
    곰피추출물의 dieckol 함량분석
Acknowledgement
국문요약
Conflict of interests
ORCID
References

• 한웅호(강원대학교 식품환경융합학과) | Xionggao Han (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea)
• 최선일(강원대학교 식품환경융합학과) | Sun-Il Choi (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea)
• 문효(강원대학교 식품환경융합학과) | Xiao Men (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea)
• 이세정(강원대학교 식품환경융합학과) | Se-jeong Lee (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea)
• 오건(강원대학교 식품환경융합학과) | Geon Oh (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea)
• 진희구(차의과학대학교 식품생명공학과) | Heegu Jin (Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Korea)
• 오현지(차의과학대학교 식품생명공학과) | Eunjin Kim (Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Korea)
• 김은진(㈜네추럴웨이) | Eunjin Kim (Naturalway Co. Ltd, Pocheon, Gyeonggi, Korea)
• 김종욱(㈜네추럴웨이) | Jongwook Kim (Naturalway Co. Ltd, Pocheon, Gyeonggi, Korea)
• 이부용(차의과학대학교 식품생명공학과) | Boo-Yong Lee (Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Korea)
• 이옥환(강원대학교 식품환경융합학과) | Ok-Hwan Lee (Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea) Corresponding Author