This study aimed to predict the shelf life of black soybean Sunsik to develop a functional labeling system for the product. The Arrhenius equation was used to calculate the shelf life by examining alterations in the dietary fiber and calcium levels of black soybean Sunsik stored at 25, 35, and 50°C for 0, 6, and 12 months. Dietary fiber and calcium analyses were performed according to the experimental methods specified in the Food Code of the Ministry of Food and Drug Safety. Both black soybean Sunsik (BS) and black soybean Sunsik containing nondigestible maltodextrin and calcium lactate (BSN) exhibited an upward trend in dietary fiber content after 12 months of storage, compared to their initial levels. During storage, the phytate in Sunsik degraded, releasing cations that facilitated the formation of new cross-links between pectic acid and middle lamella, which ultimately increased dietary fiber content. Conversely, the calcium contents of both BS and BSN decreased with prolonged storage. Based on these findings, the expected shelf life of BS and BSN was calculated as 15.65 and 28.34 months, respectively.

This study was performed to investigate the quality characteristics and antioxidant activity of black soybean Sunsik product with functional food ingredients and functional labeling system. We prepared black soybean Sunsik (BS) containing black beans, cereals, and vegetables. Black soybean Sunsik with nondigestible maltodextrin and calcium lactate (BSN) was prepared by adding non-digestible maltodextrin and calcium lactate to the base recipe to apply a functional labeling system. The particle size in BS was 118.00 μm, whereas BSN was 127.00 μm. The respective L, a, and b color values of BS were 73.25, 2.36, and 14.21. The respective L, a, and b values of BSN were 73.21, 2.36, and 14.31. The respective water retention capacities of BS and BSN were 241.67% and 216.33%. No significant difference was observed between BS and BSN in the three physicochemical properties described above. However, the pH of BSN was 5.45, which was significantly lower than that of BS. The total respective phenolic contents of BS and BSN were 1.75 mg GAE/g and 1.61 mg GAE/g, and total respective flavonoid contents of BS and BSN were 6.36 mg RE/g and 5.95 mg RE/g. The antioxidant capacities of BS and BSN were compared via assays of DPPH and ABTS radical scavenging activities, FRAP, and reducing power. The antioxidant activities of BS and BSN increased in a dose-dependant manner. No significant difference between BS and BSN was observed in any measure of antioxidant capacity. These results suggested that the addition of functional food ingredients (non-digestible maltodextrin and calcium lactate) did not affect the quality characteristics and antioxidant activity of black soybean Sunsik.

This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography–photodiode array. The results showed that the correlation coefficient of calibration curve (R2) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 μg/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91- 102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.

This study aimed to investigate the validation and modify the analytical method to determine quercetin- 3-o-gentiobioside and isoquercitrin in Abelmoschus esculentus L. Moench for the standardization of ingredients in development of functional health products. The analytical method was validated based on the ICH (International Conference for Harmonization) guidelines to verify the reliability and validity there of on the specificity, linearity, accuracy, precision, detection limit and quantification limit. For the HPLC analysis method, the peak retention time of the index component of the standard solution and the peak retention time of the index component of A. esculentus L. Moench powder sample were consistent with the spectra thereof, confirming the specificity. The calibration curves of quercetin-3-o-gentiobioside and isoquercitrin showed a linearity with a near-one correlation coefficient (0.9999 and 0.9999), indicating the high suitability thereof for the analysis. A. esculentus L. Moench powder sample of a known concentration were prepared with low, medium, and high concentrations of standard substances and were calculated for the precision and accuracy. The precision of quercetin-3-o-gentiobioside and isoquercitrin was confirmed for intraday and daily. As a result, the intra-day precision was found to be 0.50-1.48% and 0.77-2.87%, and the daily precision to be 0.07-3.37% and 0.58-1.37%, implying an excellent precision at level below 5%. As a result of accuracy measurement, the intra-day accuracy of quercetin-3-o-gentiobioside and isoquercitrin was found to be 104.87-109.64% and the daily accuracy thereof was found to be 106.85-109.06%, reflecting high level of accuracy. The detection limits of quercetin-3-o-gentiobioside and isoquercitrin were 0.24 μg/mL and 0.16 μg/mL, respectively, whereas the quantitation limits were 0.71 μg/mL and 0.49 μg/mL, confirming that detection was valid at the low concentrations as well. From the analysis, the established analytical method was proven to be excellent with high level of results from the verification on the specificity, linearity, precision, accuracy, detection limit and quantitation limit thereof. In addition, as a result of analyzing the content of A. esculentus L. Moench powder samples using a validated analytical method, quercetin-3-o-gentiobioside was analyzed to contain 1.49±0.01 mg/dry weight g, while isoquercitrin contained 1.39±0.01 mg/dry weight g. The study was conducted to verify that the simultaneous analysis on quercetin-3-o-gentiobioside and isoquercitrin, the indicators of A. esculentus L. Moench, is a scientifically reliable and suitable analytical method.

본 연구에서는 표준화된 곰피추출물의 항산화 및 콜레 스테롤 개선에 대한 효능평가를 통해 건강기능식품 소재로서의 가치를 검토하기 위해 총 폴리페놀, 총 플라보노이드 및 dieckol 함량을 측정하였으며 DPPH, ABTS radical 소거능, reducing power 및 FRAP 활성을 통하여 곰피추 출물의 in vitro 항산화 활성을 조사하였고 표준화된 곰피 추출물의 HMG-CoA reductase 저해 활성 및 세포 내 콜레스테롤 생성 억제 효능을 평가하였다. 표준화된 곰피추출물의 총 폴리페놀, 총 플라보노이드 및 dieckol 함량은 각각 9.64±0.04 mg GAE/g, 2.72±0.08 mg RE/g, 27.42±0.66 mg/g 으로 나타났다. 표준화된 곰피추출물의 in vitro 항산화활 성, HMG-CoA reductase 저해활성 및 세포 내 콜레스테롤 생성 억제 효능은 농도의존적으로 증가하는 경향을 보였으며 이는 표준화된 곰피추출물에 함유되어 있는 페놀성 화합물에 기인된 효능으로 사료되며 항산화성분, 항산화 효과, 콜레스테를 개선 효능간의 상관관계가 있음을 확인 하였다. 향후, 표준화된 곰피추출물에 대한 in vivo 모델 에서의 전임상 연구 및 작용기전 입증되면 인체적용시험을 통해 이중기능성을 갖는 건강기능식품의 개발이 가능할 것으로 사료된다.

This study was conducted to examine the antioxidative effect and nitrite scavenging ability of extract from Acanthopanax cortex shoot. The total phenolic compound and flavonoids contents of extract from Acanthopanax corex shoot were 116.33±6.09 ㎎ GAE/g and 65.07±4.10 ㎎ RE/g, respectively. Antioxidative activities were measured by various in vitro models such as DPPH radical scavenging activity, FRAP, reducing power, ABTS radical scavenging activity, ORAC assay. This results showed that the extract of Acanthopanax cortex shoot was effective in scavenging radicals and protecting oxidation when assessed various in vitro systems. Similarly, the nitrite scavenging ability of extract was increased in a dose dependent manner. Moreover, ORAC value at a concentration of 0.1 ㎎/㎖ was 103.4±5.6μM TE/g. Considering high consumer demand beneficial health effects, Acanthopanax cortex shoot can be utilized to develop functional food health-promoting and natural antioxidant agents.

이 연구의 목적은 삼림파괴로 유발되는 몇 가지 기상학적 요소의 변화를 관측을 통해서 조사하였다. 이 연구를 위해서 기상관측 장치를 숲이 파괴된 곳과 숲이 보존되어 있는 곳에 설치하여 1년간(2006. 12.-2007. 12) 연속 관측하였다. 이 후로 숲이 파괴된 지점을 point 1 그리고 숲이 보존되어 있는 지점을 point 2라고 지칭하기로 한다. 이 연구에서는 여름철에 강우가 있은 다음 날부터 1주일 동안 관측된 두 지역의 자료를 비교 분석하였다. 관측 자료의 분석을 통하여 야간에는 두 지역의 온도분포가 비슷하였지만, 낮에는 지점 1에서 관측된 기온이 지점 2보다 약 1.5˚C 높게 나타났다는 것을 확인할 수 있었다. 하지만 야간에는 두 지점간의 차이가 작았다. 상대습도도 숲의 보존지역(지점2)에서 높게 나타났는데, 그 차이는 한낮에 10%정도에 이르렀다. 지표와 지중 15cm 깊이의 온도도 두 지역 사이에 큰 차이를 보였다. 그 차는 낮에 크고 야간에는 작았는데, 대체로 지점 1에서의 값이 3-10˚C 더 높게 나타났다. 토양수분은 지점에서 7.1%, 지점 2에서 19.5%로 나타나서 지점 2에서의 토양 수분이 지점 1보다 훨씬 높았다. 두 지점에서 풍향은 주로 북서-북동풍으로 거의 차이가 없었지만, 풍속은 지점 2(0.3m/s)에서 지점 1(0.5m/s)보다 작게 관측되었다. 이러한 관측 자료를 바탕으로 두 지점 사이에 존재하는 열수지적 차이에 대해서도 분석하였다.

In this paper, lysimeter was installed to analyze the moisture fluctuations on the surface of a forest. The weight of the soil was measured, and the moisture fluctuations were calculated through the difference in weight over time. The amount of dew condensation on the surface of the ground was about 2-7 mm. January experienced the most dew condensation (7.2 mm). It was found that about 43 mm of dew condensation was generated over one year. To analyze the characteristics of evapotranspiration in the forest, the evapotranspiration on the surface was measured by the lysimeter method and the evapotranspiration on the upper part of the canopy was measured by the eddy covariance method. These results were compared and analyzed. Until mid-October, the evapotranspiration of the forest was active, and the amount of evapotranspiration on the top of the canopy was higher than the amount on the surface. Thereafter, the amount of evapotranspiration on the top of the canopy decreased due to the lowering of temperature and net-radiation. The amount of evapotranspiration on the surface and above the canopy showed the same tendency.

Background: The leaf temperature (TLEAF) is one of the most important physical parameters governing water and carbon flux, including evapotranspiration, photosynthesis and respiration. Cnidium officinale is one of the important folk medicines for counteracting a variety of diseases, and is particularly used as a traditional medicinal crop in the treatment of female genital inflammatory diseases. In this study, we developed a model to estimate TLeaf of Cnidium officinale Makino based on black globe temperature (TBGT). Methods and Results: This study was performed from April to July 2018 in field characterized by a valley and alluvial fan topography. Databases of TLEAF were curated by infrared thermometry, along with meteorological instruments, including a thermometer, a pyranometer, and an anemometer. Linear regression analysis and Student’s t-test were performed to evaluate the performance of the model and significance of the parameters. The correlation coefficient between observed TLEAF and calculated TBGT obtained using an equation, developed to predict TLEAF based on TBGT was very high (r2 = 0.9500, p < 0.0001). There was a positive relationship between TBGT and solar radiation (r2 = 0.8556, p < 0.0001), but a negative relationship between TBGT and wind speed (r2 = 0.9707, p < 0.0001). These results imply that heat exchange in leaves seems to be mainly controlled by solar radiation and wind speed. The correlation coefficient between actual and estimated TBGT was 0.9710 (p < 0.0001). Conclusions: The developed model can be used to accurately estimate the TLeaf of Cnidium officinale Makino and has the potential to become a practical alternative to assessing cold and heat stress.

The effect of elevation and meteorological elements is analyzed in Jeju Island for analysis of evapotranspiration with two different height observation station during 3 years data. The slope of temperature gradient recorded 1.30 ℃/100 m from the analysis of temperature data. There is much difference evapotranspiration between low elevation and high elevation station because of decrease solar radiation and wind speed with height increase. The evapotranspiration is observed by mini lysimeter at Hankukgonghang. The result of observation is presented 34.2% of global solar radiation changed into evapotranspiration during 4 clear days.

Obesity, a strong risk factor for the development of chronic diseases, is characterized by an increase in the number and size of adipocytes differentiated from precursor cells, preadipocytes. Recent research suggests that increased reactive oxygen species (ROS) production in 3T3-L1 adipocyte facilitates adipocyte differentiation and fat accumulation. This study was to investigate whether reduced ROS production by Sargassum micracanthum extract (SME) could protect the development of obesity through inhibition of adipogenesis. 3T3-L1 preadipocytes were treated SME for up to 8 days following standard induction of differentiation. The extent of differentiation reflected by amount of lipid accumulation and ROS production was determined by Oil red O staining and nitroblue tetrazolium (NBT) assay. Treatment of SME significantly inhibited ROS production and adipocyte differentiation that is depend on down regulation of NADPH oxidase 4 (NOX4), a major ROS generator, and peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα), a key adipogenic transcription factor. These results indicate that SME can inhibit adipogenesis through a reduced ROS level that involves down-regulation of NOX4 expression or via modulation of adipogenic transcription factor.

Recently, NADPH oxidase 4 (NOX4)-mediated generation of intracellular reactive oxygen species (ROS) was proposed to accelerate adipogenesis of 3T3-L1 cell. We have previously shown that Cheonnyuncho (Opuntia humifusa) extract significantly inhibited adipocyte differentiation via downregulation of PPARγ (peroxisome proliferator-activated receptor gamma) gene expression. In this study, we focused on the molecular mechanism(s) of NOX4, G6PDH (glucose-6-phosphate dehydrogenase) and antioxidant enzymes in anti-oxidative activities of 3T3-L1 adipocytes. Our results indicate that Cheonnyuncho extracts markedly inhibits ROS production during adipogenesis in 3T3-L1 cells. Cheonnyuncho extracts suppressed the mRNA expression of the pro-oxidant enzyme such as NOX4 and theNADPH-producing G6PDH enzyme. In addition, treatment with Cheonnyuncho extract was found to upregulate mRNA levels of antioxidant enzymes such as Mn-SOD (manganese-superoxide dismutase), Cu/Zn-SOD (copper/zinc-SOD), glutathione peroxidase (GPx), glutathion reductase (GR), and catalase, all of which are important for endogenous antioxidant responses. These data suggest that Cheonnyuncho extract may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanism(s) that involves direct down regulation of NOX4 and G6PDH gene expression or via upregulation of endogenous antioxidant responses.

In this study, 80% ethanolic extracts of tartary and common buckwheats were assessed for their total phenol content, total flavonoids content, antioxidant activity (DPPH, ABTS radical scavenging activity and reducing power), and anti-adipogenic effects. Our results show that total phenol contents of 80% ethanolic extract from tartary and common buckwheats were 17.35±0.41 and 8.20±0.28 μg GAE/g, respectively. Antioxidant activities of 80% ethanolic extract from tartary buckwheat were significantly higher than that of common buckwheat extract (p<0.05). During adipocyte differentiation, 80% ethanolic extracts of tartary and common buckwheat significantly inhibited lipid accumulation compared to control cells. We further evaluated the effect of buckwheat extracts on the changes of key gene expression associated with 3T3-L1 adipogenesis and ROS production. Tartary buckwheat extract was more suppressed the mRNA expressions (PPARγ and aP2) than that of common buckwheat extract. Moreover, tartary buckwheat inhibited the mRNA expression of both NOX4 (NADPH oxidase 4) and G6PDH (glucose-6-phosphate dehydrogenase). These results indicate that anti-adipogenesis effect of tartary buckwheat can be attributed to phenolic compound that may potentially inhibit ROS production.

Evapotranspiration is one of the important elements related water cycle and there is many kind of measurement method of evapotranspiration today. This study developed mini lysimeter for the purpose of direct measurement of evapotranspiration and installed on 5th, July, 2010 at the field of MRI which located at Tsukuba, Ibaraki, Japan for continuous measurement and understand relation between evapotranspiration and meteorological elements expecially radiation elements. And compared the evapotranspiration data of lysimeter with Bowen Ratio Method. The result of this study is as follows; There is high related with solar radiation and evapotranspiration with R2=0.947. and 46 % of solar radiation converted into evapotranspiration during clear 5 days. In net radiation also highly related with evapotranspiration, we can derive evapotranspiration is mainly controlled by radiation energy in clear days. From the 104 days data, there is only 9 % difference between Bowen Ratio Method and evapotranspiration of lysimeter which was developed from this study is very useful to estimate evapotranspiration at field site with simple and high accuracy. High accuracy and resolution measurement of evapotranspiration by lysimeter can give a chance further study of meteorological phenomena of on ground expecially in night time condensation which means abnormal energy flow.

최근 천년초 선인장은 녹차 및 양파와 같은 피토케미칼(phytochemical)에 함유된 페놀성 화합물의 중요한 소재로 보고되고 있다. 본 연구에서는 천년초 열수 및 80% 에탄올 추출물의 총 페놀 및 총 플라보노이드 함량, 항산화 활성(DPPH 라디칼 소거능 및 환원력) 및 항비만 활성을 평가하였다. 총 페놀 함량은 천년초 열수 추출물 및 80% 에탄올 추출물에서 각각 와 mg GAE/g로 나타난 반면, 총 폴라보노이드 함량은 80% 에탄올

The temporal variations of methene emission and its relation to air temperature were investigated in Upo-swamp during June 2007 through July 2008. To perform this study, the methene emission and air temperature were observed using the buoy-type chamber and automatic weather observation system(AWS), respectively. The methene emissions were much during summertime(June〜August). The maximum value(about 73.4 ㎎/㎡/hr) appeared at August. The emission diminished by degrees after August. The methene emissions were fewer from September to May of the following year. The peak value(73.4 ㎎/㎡/hr) of the methene emission is very much compared to that of rice pappy field known as about 28.7 ㎎/㎡/hr.

We need water equivalent unit data of snowfall for the purpose of forecast and hydrology related research area. This study developed new method of automatic recording snowfall as weight unit. The instrument designed for measuring weight of snowfall by stain-gauge loadcell. Field test of instrument carried out at Daegwallyeong Obs. Station from 22 Jan. to 22 Feb. 2007. During observation period there is 15.3 cm snow depth and 16.0 mm of accumulated water equivalent depth at Daegwallyeong Obs. Station on 13 to 14 Feb. 2007. But the instrument of this study recorded 22.1 mm of water equivalent depth. It is not easy to explain difference between Daegwallyeong and this study. Because this study is only one case of comparison of snow measurement and there is very little amount of snow observation research. The density of snowfall calculated from 0.09 to 0.15 g/cm3 from the observation data of 13 to 14 Feb. 2007. There is high relation between radar echo and snowfall amount measured by weight unit. It can supports forecast of snowfall and development of numerical model for forecast.

This study was performed to provide the basic information on characteristics of groundwater pollution in Gyeongsan city. Forty two groundwater samples were collected, and pH, DO, COD, NH3-N, NO3--N, T-N, PO43--P, Cl, Ca, Mg, hardness, evaporate residues and others were investigated. And, ANOVA analyses were carried out to reveal the differences in water pollution indicator values of by industry, commerce/residence and agriculture areas. The results were as follows. 1. The mean values of pH, DO, COD, NH3-N, NO3--N, T-N, PO43--P, Cl, evaporate residues, Ca and hardness were 6.9, 7.9 ㎎/ℓ, 0.4 ㎎/ℓ, 2.44 ㎎/ℓ, 2.73 ㎎/ℓ, 6.06 ㎎/ℓ, 0.82 ㎎/ℓ, 32.72 ㎎/ℓ, 381.67 ㎎/ℓ, 41.53 ㎎/ℓ, 177.17 ㎎/ℓ, respectively. 2. As groundwater became deeper, the values of Cl, Ca, Mg, Na, hardness and evaporate residues remarkably increased, but those of COD, NH3-N, NO3--N, NO2--N, T-N decreased. 3. The values of COD, Cl, Ca, Mg, Na, hardness and evaporate residues were very high in industrial area, and those of NH3-N, NO2--N, T-N were very high in commercial/residential area, and those of NO3--N were a little high in agricultural area. 4. The correlations between depth and each value of Mg, Na, Fe, hardness and evaporate residues were highly positive, and those between DO and each value of Mg, Cu, Fe, hardness and evaporate residues were highly negative. 5. According to ANOVA analyses, the differences in three area groups (industry, commerce/residence and agriculture) on the values of NH3-N, T-N, evaporate residues, hardness, Ca, Mg, K and Fe were significant at 1% level.