전통 된장의 숙성기간 중 기능적 특성의 변화를 조사하기 위하여 상황버섯 추출물을 이용하여 된장을 담금하여 일정기간 숙성한 후, 된장의 protease 활성, 항돌연변이 활성, isoflavone 등을 측정하였다. Protease의 활성은 상황버섯 추출물을 이용한 실험구가 3.15 unit/mL로 대조구보다 높은 활성을 나타내었으며 tyrosinase의 저해 활성과 ACE 저해 활성은 실험구가 각각 45.78%와 55.18%로 대조구보다 약 20%
To evaluate changes in functional characteristics of traditional Doenjang during aging, Doenjang was prepared using an extract of Phellinus linteus (Phellinus extract). Control Doenjang was aged without the extract. The protease activity of Doenjang prepared with Phellinus extract was 3.15 units/mL. Tyrosinase and acetylcholinesterase inhibition activities were 45.78% and 55.18% of control, respectively, in the treated sample. When Salmonella enterica serovar Typhimurium TA100 was used as a reporter strain, antimutagenic activities against the mutagens MNNG and NPD were 90.42% and 82.57% of control values in the treated sample. When S. enterica serovar Typhimurium TA98 was used, antimutagenic activities were 60.28% and 50.33% of control, respectively. Hydrogen-donating activity was 86.65% in the treated sample, which was higher than that of the control (61.69%). Daidzin (an isoflavon glucoside) levels in Doenjang prepared with Phellinus extract were higher, by 35.49 mg/kg, than the control, whereas genistin was not detected in either group. Daidzin and genistin aglycone levels were 263.01 mg/kg and 262.60 mg/kg in the control and test groups, respectively.