본 실험은 메밀새싹의 추출조건에 따른 수율, 총 페놀, -glucosidase 저해활성능, 및 ACE 저해활성능에 대해 반응표면 분석법을 이용하여 추출조건을 최적화하였다. 중심합성계획에 따라 추출온도(), 추출시간(0~12 hr) 및 에탄올 농도(0~100%)를 달리하였을 때 반응 표면 회귀식의 R2는 수율, 총 페놀, -glucosidase 저해활성능 및 ACE 저해활성능에서 각각 0.9461(p-glucosidase 저해활성능 및 ACE 저해

Response surface methodology (RSM) was used to optimize extraction conditions for functional components of buckwheat (Fagopyrum esculentum). A central composite design was applied to investigate the effects of three independent variables, namelyextraction temperature (X1), extraction time (X2), and ethanol concentration (X3), on responses including extraction yield (Y1), total phenolic content in the extract (Y2), -glucosidase inhibition activity (Y3), and acetylcholine esterase (ACE) inhibition activity (Y4). Data were analyzed using an expert design strategy and statistical software. The maximum yield was 24.95% (w/w) at extraction temperature, 8.75 hextraction time, and 15.65% (v/v) ethanol. The maximum total phenolic yield was 222.45 mg/100 g under the conditions of extraction temperature, 8.65 h extraction time, and 81.72% (v/v) ethanol. The maximum -glucosidase inhibition activity was 85.38% at , 7.86 h, and 57.58% (v/v) ethanol. The maximum ACE inhibition activity was 86.91% under extraction conditions of , 4.86 h, and 44.44% (v/v) ethanol. Based on superimposition of a four-dimensional RSM with respect to levels of total phenolics, -glucosidase inhibition activity, and ACE inhibition activity, obtained under various extraction conditions, the optimum ranges of conditions were an extraction temperature of , an extraction time of 2-8 h, and an ethanol concentration of 30-80% (v/v).

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