본 연구에서는 프로폴리스의 다양한 효능을 이용한 식품 소재 개발을 위해 반응표면분석을 이용하여 프로폴리스의 에탄올 추출농도(50, 60, 70, 80, 90%)와 추출시간에 따른 항산화능, 플라보노이드 등의 품질특성을 조사하였다. 총 페놀성 화합물 함량은 에탄올 농도가 높을수록 증가하다가 80% 이상에서는 감소하는 것으로 나타났으며, 추출시간보다는 에탄올 농도에 더 큰 영향을 받는 것으로 나타났다. 추출물의 전자공여능은 에탄올 농도가 높을수록,

A central composite design was used to optimize extraction of propolis materials using ethanol. The independent variables in extraction experiments were ethanol concentration (50, 60, 70, 80, 90%, v/v) and extraction time (1, 2, 3, 4, and 5 h). Higher ethanol concentration and shorter extraction time increased total polyphenol content, but total polyphenol concentration began to decrease when ethanol concentration was higher than 80% (v/v). Ethanol concentration was more important than extraction time in optimization of total polyphenol content in propolis extracts. Electron-donating ability increased with ethanol concentration and shorter extraction time, with ethanol concentration being of greater significance. Antioxidant ability in extracts was optimal at an ethanol concentration of 65 - 75% and with an extraction time of 2.2 - 3.6 h. Nitrite-scavenging ability was increased with use of higher ethanol concentration and shorter extraction time. Total flavonoid content was maximized with an ethanol concentration of 68 - 82% and an extraction time of 2.4 - 3.7 h. Total flavonoid content was affected by both ethanol concentration and extraction time. By superimposition of contour plots, an ethanol concentration of 72 - 82% and an extraction time of 2.2 - 3.3 h were optimal for preparation of propolis extracts.

• 김성호
• 김인호
• 강복희
• 이경희
• 이상한
• 이동선
• 김소미
• 허상선
• 권택규
• 이진만