The leaves of Allium victorialis (AV) are known an edible perennial herb, which has been used in Korean traditional medicine. However, the beneficial pharmacological effects of AV extracts (AVE) on the antioxidant activity and atopic dermatitis (AD) have not been thoroughly examined. Therefore, the present study aims to investigate both antioxidant activity and anti-inflammatory effect of AVE on AD in vitro and in vivo. Antioxidant activity was evaluated by total polyphenol content and ferric reducing ability. AVE showed a level of polyphenol content and reducing power activity. The five-week-old BALB/c mice were used as an AD-like mouse model by treating them with 1-chloro-2, 4-dinitrobenzene (DNCB). Topical administration of AVE for 3 weeks to DNCB-treated mice significantly alleviated clinical skin lesion dermatitis severity and epidermal thickness. Histopathological analysis also demonstrated that AVE decreased eosinophil and mast cell infiltration into skin and ear tissue. These results suggest that topical application of AVE inhibits the development of AD-like skin lesion in mice by their antioxidant activity. Thus, AVE may be a potential therapeutic agent for AD.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Preparation of Allium victorialis leaf extract
    Measurement of total polyphenol content
    Reducing power assay
    Experimental animals
    Experimental study with AVE for AD mice
    Measurement of ear thickness and organ weight
    Histological analyses
    Statistical Analysis
Results
    Determination of total Polyphenol contents of AVEand its reducing power activity
    AVE suppressed AD skin symptoms in DNCB-inducedmice
    Histological analysis of DNCB-induced mice skin andear lesions
DISCUSSION
COMPETING INTERESTS
ACKNOWLEDGMENTS
REFERENCES

• Hyeryeon Yang(College of Veterinary Medicine, Gyeongsang National University)
• Jong Bae Seong(College of Veterinary Medicine, Gyeongsang National University)
• Hyun Sik Yoon(College of Veterinary Medicine, Gyeongsang National University)
• Il Rae Rho(Institutes of Agriculture and Life Science, Gyeongsang National University)
• Du Hyeon Hwang(College of Veterinary Medicine, Gyeongsang National University)
• Euikyung Kim(College of Veterinary Medicine, Gyeongsang National University, Institutes of Animal Medicine, Gyeongsang National University)
• Changkeun Kang(College of Veterinary Medicine, Gyeongsang National University, Institutes of Animal Medicine, Gyeongsang National University) Corresponding Author