논문 상세보기
pp.92-96
시판 식혜에는 설탕과 프룩토오스, 글루코오스, 말토오스 및 여러 사이즈의 말토올리고당, 한계덱스트린이 함유되어 있다. 그 중 한계덱스트린은 0.09%, 밥알은 0.2%를 나타냈다. ^1H-NMR분석 결과, 한계덱스트린은 α-1, 4-결합 및 α-1, 6-결합이 15 : 1 의 비율을 나타냈다. Pullulanase 처리로, 말토오스에서 글루코오스 10 잔기 이상의 말토올리고당까지 다양한 분포를 나타냈다. 한계덱스트린은 여러 아밀라아제 처리 결과, 전통 식혜보다 가수분해율이 훨씬 높았다. α-글루코시다아제와 타액 α-아밀라이제를 공동작용시킨 경우는 62% 가수분해되었다. 그러나, 밥알의 가수분해율이 매우 낮았다. 그래서 전통 식혜에 비해 한계덱스트린의 비피두스인자로서의 효과는 적고, 밥알의 식이섬유 작용은 커졌다.
A Korean commercial sweet rice drink "Sikhye" showed sucrose, fructose, glucose, maltose, limit dextrin and various size of maltooligosaccharides in HPLC and TLC analysis. Commercial Sikhye was found to contain 0.09% of limit dextrin and 0.2% of rice residue. Limit dextrin in commercial Sikhye showed both signal of α-1, 4 and α-1, 6-glucosidic linkage with its estimation ratio of 15 : 1 by ^1H-NMR analysis. This limit dextrin was hydrolyzed to proeuce various size of maltooligosaccharides with more longer chain than that of traditional Sikhye by pullulanase. Limit dextrin was digested with enzymes(30units/ml) of α-amylase, α-glucosidase and glucoamylase from Aspergillus awamori, sweet potato β-amylase and human salivary α-amylase at 37℃ for 1 hour, respectively. Hydrolysis rates of these amylases on it were higher than in case of traditional Sikhye. α-Glucosidase plus human salivary α-amylase hydrolyzed it to 61.3%. Hydrolysis rates of these amylases on rice residue were lower than that of traditional Sikhye. These results suggest that limit dextrin in commercial Sikhye is less effective than isomaltool-igosaccharides in traditional Sikhye as a growth factor for Bifidobacterium while rice residue in commercial Sikhye is more effective than that in traditional Sikhye as dietary fiber.
