효소반응과 부분 산가수분해 결과를 해석하여 Leuconostoc mesenteriodes M-12 덱스트란수크라제 억셉터 반응 산물이 새로운 분지올리고당의 구조를 확인하였다. 분지올리고당 B_4의 구조는6^2-O-α-D-kojibiosylmaltose인 것으로 확인되었으며, 분지올리고당 B_5의 구조는 6^3-O-α-D-kojibiosylpanose였다. 억셉터 반응산물을 덱스트라나제로 분해한 결과 새로운 올리고당인 D_4를 확인할 수 있었다. 억셉터 반응의 산물을 억셉터로 이용한 두 번째 억셉터 반응의 생성물을 덱스트라나제 처리하여 D_4를 얻었는데 덱스트라나제와 글루코아밀라제에 의해 분해되지 않았다. 그 구조는 6^2-O-α-D-kojibiosylisomaltose로 확인되었다. 직선상 또는 분지결합을 가진 d.p. 6이하의 억셉터 반응산물의 생성 패턴도 확인하였다.

The structures of novel branched oligosaccharidse synthesized by the acceptor reaction with dextransucrase from Leuconostoc mesenteriodes M-12 were proposed in accordance with the results obtained from enzymatic hydrolysis and a partial acid hydrolysis. The structure of branched oligosaccharide B_4 was shown to be 6^2-O-α-D-kojibiosylmaltose. Branched oligosaccharide B_5 was shown to be 6^3-O-α-D-kojibiosylpanose. By reacting the acceptor reaction products with endodextranase a novel branched oligosaccharide(D_4) coule be produced. D_4 was derived from the result of endodextranase hydrolysis of oligosaccharides synthesized by the second acceptor reaction with dextransucrase and was resistant to endodextranase and glucoamylase. The proposed structure of D_4 was 6^2-O-α-D-kojibiosylisomaltose. Formation pattern of the acceptor reaction products smaller than d.p. 6 with linear or branched linkage was also shown.

• 배만종 | Man Jong Bae
• 손규목 | Gyu Mok Son
• 성태수 | Tae Soo Sung