멥쌀식혜와 찹쌀식혜의 한계덱스트린을 알코올침전, Biogel P-2의 겔 크로마토그래피, Superose 12 겔 크로마토그래피 컬럼을 사용한 FPLC로 정제하여 ^1H-NMR 분석을 행하였다. 멥쌀식혜의 한계덱스트린은 α-1, 4-글루코시드 결합과 α-1, 6-글루코시드 결합의 비율이 1:4.5, 찹쌀식혜의 한계덱스트린은 1:5.9를 나타냈다. Pullulanase 소화로 멥쌀식혜 및 찹쌀식혜의 한계덱스트린은 말토오스, 말토트리오스, 말토테트라오스, 말토펜타오스, 말토헥사오스까지 나타내 이들이 서로 조합하여 한계덱스트린을 만들고 있는 것으로 분석되었다.

Bench scale Sikhyes were produced from rice and glutinous rice and limit dextrins in rice Sikhye and glutinous rice Sikhye were purified by ethanol precipitation and Biogel P-2 gel chromatography and FPLC on Superose 12 column and analyzed. The purified limit dextrin in rice Sikhye and glutinous rice Sikhye showed both signal of α-1, 4- and α-1, 6-glucosidic linkage with its estimation ratio of 4.5 : 1 and 5.9 : 1, respectively, by ^1H-NMR analysis. Limit dextrins were hydrolyzed by pullulanase. The enzyme hydrolysis products contained maltose, maltotriose, maltotetraose, maltopentaose and matohexaose. These results suggest that limit dextrins were composed of these maltoolgosaccharide series with α-1, 6-glucosidic bond.

• 방병호 | Byung Ho Bang
• 양철영 | Cheul Young Yang
• 이상건 | Sang Gun Lee