Protein can be provided by cultivating various microbes, which contain more than 30% protein content by cell dry weight. This study compared intracellular protein concentrations of various wild-type yeasts from different sources to select the best yeast strain with high protein concentration. Among them, Saccharomyces cerevisiae KCCM 34709, used for molasses fermentation, exhibited 4.1-fold higher protein concentration than a laboratory yeast strain, S. cerevisiae D452-2. In this study, an approach consisting of random mutagenesis coupled with the Bradford protein assay-based screening method was applied to enhance the S. cerevisiae KCCM 34709 protein content. Among 1,000 mutants, the #180 mutant strain produced 5,041±519 mg/L total amino acid in 48 h, which was 31% higher than the parental S. cerevisiae KCCM 34709 strain. These results demonstrate that the #180 mutant strain can be an attractive cell factory for animal-free protein production.

Abstract
서 론
재료 및 방법
    사용 균주
    96-well plate 수준 효모 배양
    플라스크 수준 효모 배양
    단백질 농도 측정
    자외선 조사를 통한 돌연변이 라이브러리 구축
    통계 분석
결과 및 고찰
    단백질 고함량 식용 효모 균주 선별
    S. cerevisiae KCCM 34709 균주의 돌연변이 라이브러리 구축 및 1차 스크리닝
    단백질 고함량 변이체 선별을 위한 2차 스크리닝 및 아미노산 프로파일링
요 약
감사의 글
References
Author Information

• 전기범(중앙대학교 식품공학부) | Gi-Beom Jeon (Department of Food Science and Technology, Chung-Ang University)
• 이영오(신세계푸드 연구개발팀) | Young-Oh Lee (R&D Team of Better Meat, Shinsegae Food Inc.)
• 주범진(신세계푸드 연구개발팀) | Bumjin Joo (R&D Team of Better Meat, Shinsegae Food Inc.)
• 진영욱(한국식품연구원 전통식품연구단) | Young-Wook Chin (Traditional Food Research Group, Korea Food Research Institute)
• 김선기(중앙대학교 식품공학부) | Sun-Ki Kim (Department of Food Science and Technology, Chung-Ang University) corresponding author