This study was conducted to determine seafood product quality change in the fresh food cold chain logistics. Salmon was stored in three different simulated refrigerated distribution environments according to door opening time (0, 20, and 60 s) every 10 min before the samples’ qualities were evaluated. The temperature inside the refrigerator generally rose from the bottom to the top, and it was possible to confirm the rapid change in temperature in the order of A, B, and C as the door opening time increased. It was observed that the freshness of salmon decreased as the door opening time increased through the evaluation of various food qualities of salmon, such as its appearance, color, pH, VBN, moisture content, hardness, general bacteria, and E. coli bacteria. It was confirmed that fresh food quality is strongly associated with delivery temperature, while cold-chain delivery vehicles must reduce heat exposure time during delivery.