The characterization of nuclear materials is crucial for global nuclear safeguards efforts, as these materials can potentially be used for illicit purposes. In this study, we evaluated the applicability and performance of the In-Situ Object Counting System (ISOCS) equipment for the characterization and quantification of uranium, including uranium pellets and radioactive wastes. Our methodology involved using ISOCS to measure samples with different enrichments and total amounts of uranium, and to analyze the results in order to evaluate the ISOCS’s effectiveness in accurately characterizing the various uranium samples. To this end, we compared the ISOCS results with those of the Multi-Group Analysis for Uranium (MGAU) system, which is currently used in the field of international safeguards. The results of this study showed that the ISOCS was sensitive enough to analyze small amounts of uranium pellet, with %differences ranging from -0.7% to 19%. However, when analyzing shielded nuclear materials like in concrete waste, the uncertainty was relatively high, with %differences ranging from 11% to 67%. On the other hand, the MGAU system was unable to analyze uranium for the same spectrum, indicating the superiority of the ISOCS in terms of usability. The ISOCS instrument was also found to be effective in analyzing uranium in various types of samples without the need of standard sources. Overall, the findings of this study have important implications for the development of more effective safeguards strategies for the characterization of nuclear materials. The ISOCS instrument could be a reliable tool for analyzing nuclear materials, contributing to global safeguards efforts to reduce the risk of nuclear proliferation.