Hangovers, resulting from excessive alcohol intake, manifest hours after drinking, causing symptoms like thirst, headache, and fatigue. Alcohol is metabolized in the liver by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), with acetaldehyde and reactive oxygen species contributing to toxic effects. Morning Care (MC) products were evaluated in male and female mice to assess their impact on alcohol metabolism and hangover alleviation. The study revealed that pre-administration of MC products led to a significant reduction in blood ethanol and acetaldehyde concentrations postalcohol ingestion. This remarkable finding suggests a potential breakthrough in hangover relief. Enhanced ADH and ALDH activities were observed in blood and liver samples, indicating improved alcohol metabolism. Interestingly, gene expression levels of ADH and ALDH in the liver did not show significant differences, suggesting that MC products likely enhance enzyme activities through post-translational modifications rather than altering gene expression. These findings underscore the potential of MC products to mitigate hangover symptoms by enhancing alcohol metabolism.

Introduction
Material and Methods
    Food item manufacturing report
    Animal tests
    Ethanol assay
    Assays of ADH and ALDH activities in liver of SD rats
    Determination of relative expression of ADH and ALDH genes using real-time PCR
    Statistical analysis
Results
    Analysis of blood ethanol and acetaldehyde concentrations
    Expression of the ADH and ALDH genes in the liver of male and female mice
Discussion
Acknowledgments
Author Contributions
Declaration of Interests
References
Author Information

• Chanjin Yoon(Department of Molecular and Life Science, Hanyang University, Institute of Natural Science & Technology, Hanyang University)
• Hyo Keun Kim(Department of Molecular and Life Science, Hanyang University)
• Yu Seong Ham(Department of Molecular and Life Science, Hanyang University)
• Chul-Su Yang(Department of Molecular and Life Science, Hanyang University, Department of Medicinal and Life Science, Hanyang University) Corresponding author