Moringa oleifera, a versatile plant, has been traditionally used to treat various ailments and is gaining scientific attention due to its potential as a medicine. Native to the Indian subcontinent, it is widely grown in tropical and subtropical regions, thriving in Asia, Africa, and South America, especially in arid climates. This study explores the antioxidant potential of Moringa oleifera leaf extract (MOLE), employing a comprehensive screening approach with various solvents to identify the most effective extraction method. Initial experiments assessed antioxidant efficacy and yield using distilled water (D.W.), 95% ethanol, and 95% methanol. Among these, 95% ethanol extract demonstrated superior antioxidant activity, confirmed through assays such as 2,2-diphenyl-1-14 picrylhydrazyl (DPPH) radical scavenging assay, total polyphenol content analysis, and reducing power assay. In addition, with the 95% ethanol MOLE, a higher extraction efficiency was yielded compared to other solvents, making it the most effective for large-scale preparation. HPLC analysis revealed the presence of key bioactive compounds, including ellagic acid, rutin, Q-3-O, quercetin, and kaempferol. Results revealed that MOLE, prepared using 95% ethanol, exhibited remarkable antioxidant properties, attributed to its rich polyphenolic content. This research underscores the therapeutic potential of MOLE as a natural antioxidant source and highlights the importance of solvent optimization in phytochemical extractions.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Extraction process of Moringa oleifera leaf extract(MOLE)
    2,2-diphenyl-1-14 picrylhydrazyl (DPPH) radicalscavenging assay
    Reducing power assay
    Measurement of total polyphenol content
    Quantification of chemical content in MOLE usingHPLC analysis
    Statistical analysis
RESULT
    Determination of antioxidant activity of MOLE withsuitable solvent
    Determination of radical scavenging activities of MOLE
    Determination of total ferric reducing ability of MOLE
    Determination of extraction yield
    Determination of chemical content in MOLE using HPLCanalysis
DISCUSSION
REFERENCES

• Most Nusrat Zahan(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Donghyeon Lee(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Jimin Park(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Sobin Jeon(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Ye Rin Kim(Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea)
• Il Rae Rho(Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea)
• Euikyung Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea, Institute of Animal Medicine Gyeongsang National University, Jinju 52828, Korea)
• Changkeun Kang(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea, Institute of Animal Medicine Gyeongsang National University, Jinju 52828, Korea) Corresponding author