Maturity and spawning of the Octopus longispadiceus was investigated based on the samples collected in the East Sea. We analyzed monthly changes in maturity stage, gonadosomatic index (GSI), eggs diameter, fecundity and body weight at group maturity. Mature stages appeared every month, and GSI values were high every month. Spawning season was estimated to be year-round, and main spawning occurs intensively in sping and fall. The eggs diameter of mature stage was 19.2 ± 3.0 mm. The total number of eggs in the fecundity ranged from 31 eggs in 189 g (BW) to 184 eggs in 1,013 g (BW), which was proportional to body weight (BW), and the relationship between total weight and fecundity was F = 0.7593 × BW0.7719 (R2 = 0.7462). The percentages of sexually mature females estimated using a logistic equation were over 50% at 323.4 g BW, 75% at 451.4 g BW and 97.5% at 750.3 g BW, respectively. We think that it be good way for Octopus longispadiceus to be regulated by fishing gear and fishing method.