This study investigated the effects of different emulsification techniques—high-pressure homogenization (HPH) and ultrasonic homogenization (USH)—on the properties of β-glucan-loaded liposomes under varying operational conditions. The results showed that, in HPH, increasing both the pressure (5,000–15,000 psi) and the number of cycles (1–3) gradually reduced the average particle size of the liposomes, with all particles remaining below 200 nm and a polydispersity index (PDI) under 0.1, with no significant differences (p>0.05). Liposomes produced with USH at the lowest energy level (20% amplitude for 1 min) exhibited the largest average particle size (594.63 nm) and greatest size variation. However, increasing the power and duration of USH reduced both the liposome size and PDI, making them comparable to those produced by HPH. Turbidity improved with increasing energy in both methods (p<0.05), although the turbidity ranges differed: HPH ranged 0.243–0.839 and USH, 0.628–1.715. Overall, this study demonstrates that HPH is more effective than USH in producing stable β-glucan liposomes.

Abstract
서 론
재료 및 방법
    실험재료
    느타리버섯 균주 배양 및 베타글루칸 추출
    느타리버섯 추출물의 베타글루칸 함량
    베타글루칸 리포좀 제조
    입자특성 관찰
    탁도 측정
    통계처리
결과 및 고찰
    균질 방법과 조건을 달리한 베타글루칸 리포좀의 입자크기와 다분산지수
    균질 방법과 조건을 달리한 베타글루칸 리포좀의 제타전위와 탁도
    저장 기간에 따른 베타글루칸 리포좀의 특성
요 약
References

• 이혜윤(제주대학교 식품생명공학과) | Hye-Yoon Yi (Department of Food Bioengineering, Jeju National University, Jeju 63243, Korea)
• 천지연(제주대학교 식품생명공학과) | Ji-Yeon Chun (Department of Food Bioengineering, Jeju National University, Jeju 63243, Korea) Corresponding author
• 이강현(티엠씨와이드) | Kanghyun Lee (TMC WIDE, Jeju 63254, Korea)