This study evaluated the effects of the cells alive system (CAS) freezing method and different thawing methods (refrigeration and running water) on the quality of aged Hanwoo shoulder clods. Thawing time was significantly influenced by the thawing method, with running water thawing taking 3 h and refrigeration thawing exceeding 20 h. CAS freezing yielded significantly higher lightness (L*) values during running water thawing than it did during general freezing (p<0.05), likely owing to reduced water loss. CAS-frozen meat exhibited lower volatile basic nitrogen levels and thawing loss (2.05%–2.11%) compared with that of general frozen meat (3.17% –3.79%), indicating superior freshness and reduced quality deterioration (p<0.05). Among the textural properties, springiness was highest in frozen and running water-thawed CAS meat (10.5 mm). In contrast, general freezing combined with refrigeration thawing caused the greatest quality degradation, as reflected by the lowest pH (5.45) and water holding capacity (86.36%), and highest cooking loss (32.98%). These results suggest that CAS freezing combined with running water thawing minimizes changes in quality and enhances springiness, making it an optimal approach for the production of high-quality aged meat.