This study investigated how extraction temperature (60, 90, and 120℃) affected the yield and functional properties of mealworm (Tenebrio molitor) larvae oil (MO). Extraction yield was comparable at 60 and 90℃ but declined at 120℃, while oil purity consistently exceeded 99.5%. MO was rich in unsaturated fatty acids, especially oleic and linoleic acids, with the highest total unsaturated fatty acid content observed at 90℃. Tocopherol content peaked at 60℃, suggesting temperature-sensitive preservation of these bioactive compounds. Antioxidant activity was greatest at 90℃ and correlated with flavonoid content. Oxidative stability during storage was enhanced at 4℃ but diminished at 25℃, with higher antioxidant levels improving this stability. MO demonstrated dose-dependent antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes. Furthermore, MO showed no cytotoxicity in RAW 264.7 cells and significantly inhibited both nitric oxide production and tyrosinase activity. These findings indicate that MO holds promise as a functional ingredient for food and cosmetic applications, with 90℃ being optimal for overall functionality and 60℃ for maximizing tocopherol preservation.

Abstract
서 론
재료 및 방법
    1. 실험재료
    2. 수율 및 품질 분석
    3. 지방산 조성분석
    4. 토코페롤 분석
    5. 총 페놀 및 총 플라보노이드 함량 분석
    6. DPPH 및 ABTS 라디칼 소거활성 측정
    7. Hydroxyl 라디칼 소거활성 측정
    8. 색도
    9. 산가 및 과산화물가 분석
    10. 세포독성시험(MTT assay)
    11. 항염증 효능평가(NO assay; Griess reaction)
    12. Tyrosinase 저해능 측정
    13. 항균활성 평가
    14. 통계처리
결과 및 고찰
    1. 수율 및 품질분석
    2. 지방산 조성 및 토코페롤 분석
    3. 총 페놀 및 플라보노이드 함량과 항산화 활성
    4. 저장 중 산화 안정성
    5. 항염증 및 미백 활성
    6. 피부 상재균 및 여드름균에 대한 항균 활성
요약 및 결론
References

• 임윤지(전남농업기술원 곤충잠업연구소 농업연구사) | Yun-Ji Lim (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea) Corresponding author
• 김선암(전남농업기술원 곤충잠업연구소 농업연구사) | Sun-am Kim (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea)
• 김성연(전남농업기술원 곤충잠업연구소 농업연구사) | Seong-Yeon Kim (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea)
• 오상아(전남농업기술원 곤충잠업연구소 농업연구사) | Sang-a Oh (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea)
• 김지수(전남농업기술원 곤충잠업연구소 농업연구사) | Ji-Soo Kim (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea)
• 이주영(전남농업기술원 곤충잠업연구소 농업연구사) | Ju-Yeong Lee (Researcher, Insect and Sericultural Research Institute of Jeollanamdo Agricultural Research and Extension Services, Jangseong 57214, Korea)
• 이광우(전남대학교 융합식품바이오공학과 박사과정) | Gwang-Woo Lee (Ph.D. Student, Dept. of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea)
• 남승희(전남대학교 융합식품바이오공학과 연구교수) | Seung-Hee Nam (Research Professor, Dept. of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea)
• 문제학(전남대학교 융합식품바이오공학과 교수) | Jae-Hak Moon (Professor, Dept. of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea)