논문 상세보기
pp.151-156
한우의 수정란을 보존하기 위하여 고급육계통과 다유계통의 공란우를 선발하여 과배란처리에 따른 황체의 반응, 황체수에 대한 채란된 수정란의 수 그리고 채란 수정란수에 대한 이식가능한 수정란 생산에 미치는 영향을 조사하였다. 다유계통의 공란우는 이유시 체중에 대한 육종가 상위 20%이내, 고급육 계통의 공란우는 육질에 대한 육종가 상위 20%이내의 종빈우를 선발하였다. 과배란처리 방법은 발정후 11일차에 PEG 30% FSH를 견갑부에 피하주사로 1회 투여하
The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGFa was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.
