Sweet persimmon, ‘Fuyu’, is the major cultivar for MA storage, but browning of blossom end part and fruit surface darkening occur during storage and decrease fruit qualities in fresh fruit market. Calcium (Ca) has a very important role in cell membrane and reduces Ca-related fruit disorder. Therefore, this study was conducted to investigate the effect of soluble Ca fertigation and foliar applications on soil chemical properties, root activity, and leaf nutrient status. Ca concentration in the soil was higher in both Ca fertigation (Ca-FG) and Ca+IBA fertigation (Ca+IBA) treatments than the other treatments, such as control (Cont), Ca foliar application (Ca-FA), and IBA fertigation (IBA). The increase in soil Ca improved soil pH. The Ca+IBA treatment increased root activity. Leaf Ca concentration was significantly increased by the CA-FA application, followed by Ca+IBA, and Ca-FG treatments.