The purpose of this study was to investigate the effects of hip extension velocity (7.5 degree/second, 30 degree/second) on the relative onset time of the gluteus maximus in relation to the hamstring during hip extension in prone position. Thirteen healthy male subjects (mean age=22.6 years [SD=1.8], mean weight=73.4 kg [SD=10.3], mean height=176.1 cm [SD=6.3]) voluntarily participated in this study. Electromyographic data was collected on the gluteus maximus and hamstring to determine onset time. Statistical analyses were performed with the paired t-test. The results showed that the onset time of the hamstring was significantly faster than that of the gluteus maximus in both fast and slow hip extension velocity. The gluteus maximus began contraction .079 seconds later following the contraction of the hamstring. The onset time of the hamstring was significantly faster in fast hip extension velocity compared with slow hip extension velocity. In conclusion, it was determined that the onset time of the gluteus maximus was faster with fast hip extension velocity compared with slow hip extension velocity. There was a statistically significant difference between the onset times of the gluteus maximus and hamstring in relation to the two velocities (p<.05). Further study is needed to examine whether the velocity of hip extension can influence the onset time in a similar fashion in patients with low back pain.