산업식품공학 제25권 제4호 (p.318-324)

A Research on the Diversity and Enzyme Productivity of the Salt-Resistant Microorganisms Isolated from the Nakdong River Estuary Wetland for the Search of Fermentation Strains

키워드 :
Enzyme,Wetland,Salt-resistant,Microorganism,Industrial application

목차

Abstract
Introduction
Materials and Methods
   Isolation and cultivation of halotolerant bacteria
   Phylogenetic analysis of 16S rDNA sequence
   Hydrolytic enzyme activity analysis
   Auxin detection analysis
Results and Discussion
   Isolation of halotolerant bacteria
   Phylogenetic analysis of 16S rDNA sequence
   Hydrolytic enzyme and auxin activity analysis
Summary
References

초록

The growth characterization and enzyme productivity of halotolerant microorganisms isolated from the Nakdong River estuary wetland in Korea were investigated in this study. The isolation of halotolerant microorganisms was carried out through their cultivation at 37oC for several days using a marine agar medium. After a single-colony isolation, 16 pure colonies were obtained, and phylogenetic analysis was done using 16S-based ID service in the EzBioCloud database. The isolated strains were divided into 3 phyla, 5 families, 7 genera, and 13 species. Possible industrial applications of the strains were confirmed upon testing for amylolytic, lipolytic, and proteolytic activity to confirm the possible production of hydrolytic enzymes, growth at a pH range of 5 to 9, and various salt concentrations being tested. Thirteen strains possessed at least one enzyme activity, and 5 strains possessed 2 enzyme activities. Eight strains could tolerate up to 10% NaCl concentration. This result indicated that isolated strains had shown the possible application in food and cosmetic industries. Therefore, this study would contribute to securing domestic biological resources and improving hydrolytic enzyme activity using these strains.