Dental caries is one of the most common infectious diseases. Although its prevalence has declined significantly in many countries, dental caries in Korea remains a public health concern. The essential process in dental caries involves demineralization of the tooth surface due to a lowered pH in dental plaque, which contains various microorganisms. Streptococcus mutans is believed to play a major role in the development of dental caries by producing high concentrations of organic acids. This study aimed to evaluate the cariogenic potential of the lytic enzyme L27, derived from Bacillus licheniformis YL-1005, isolated from Korean soil. Enzyme L27 was not cytotoxic to normal periodontal ligament cells at concentrations below 250 μg/mL and had minimal impact on the pH changes caused by S. mutans . Calcium ion release occurred slowly and progressively during the first 20 minutes, but demineralization was significantly inhibited by L27. The attachment of S. mutans to hydroxyapatite beads and the reduction in surface hardness were also significantly inhibited, although the rate of bacterial attachment decreased over time. Taken together, these findings suggest that the lytic enzyme L27 from soil-derived bacteria exhibits anticariogenic effects by preventing bacterial attachment to the enamel surface and inhibiting demineralization.

Alcohol is metabolized to acetaldehyde and acetate mainly by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). Acetaldehyde accumulation is a key factor in hangover symptoms and alcohol-induced toxicity. In this study, we have investigated the effects of a Morning Care series on ethanol and acetaldehyde metabolism in male and female mice. Blood ethanol and acetaldehyde concentrations, ADH and ALDH activities in the blood and liver, and hepatic gene expression were measured at multiple time points after ethanol administration. EX PREMIUM, a hangover-relief beverage developed by Dong-A Pharm, significantly reduced blood ethanol and acetaldehyde concentrations and enhanced ADH and ALDH activities, accompanied by the upregulation of ADH and ALDH gene expression. These effects were consistently observed in both sexes. These results have suggested that EX PREMIUM effectively promotes alcohol metabolism, facilitates acetaldehyde clearance, and may contribute to alleviating alcohol-induced toxicity and hangover symptoms.

본 연구는 효소 처리 방법을 적용하여 제조한 생강 착즙박 추출물 의 수용화 특성 및 유용성분 추출 수율을 증진시키는 최적 효소 처리 조건을 선정하고, 대용량 생산 적용 가능성을 확인하고자 하였다. 효 소 처리에 사용한 효소는 세포벽 분해효소인 P와 전분분해효소인 T 를 이용하였고, P 처리 방법 및 가수 방법을 변형하여 진행하였다. 효소 처리 및 추출 조건에서는 CON 대비 GP3에서 WSI는 2배 이상 증가하였고, TP 및 TF 함량은 각각 1.6, 1.4배 증가하였다. 6-G 및 Total 함량은 1.7배 증가하는 것으로 나타났다. 효소 전처리 방법에 서는 GP3에서 TP 함량 값이 가장 높았고, 6-G 및 Total 함량 또한 유의적으로 가장 높게 나타났다. 효소 전처리 시간에서는 P를 24시 간 전처리했을 때 TP, TF, 6-G 및 Total 함량에서 유의적으로 가장 높게 나타나 효소 전처리 최적 조건을 선정하였다. 선정된 효소 전처 리 기술(25℃, 24시간)을 활용하여 생강 착즙박 추출물을 용량별(0.1, 15 kg)로 제조하여 비교한 결과, WSI는 CON 대비 효소 전처리구 (GP3)와 효소 처리구(GP1) 모두 증가하였고, 처리 용량에 따라서도 같은 경향을 나타냈으며 GP3가 가장 높았다. WAI는 대용량으로 처 리했을 때 GP3가 가장 낮았다. TP는 소용량 및 대용량 모두 CON 대비 GP3가 가장 높았고, TF는 소용량에서는 CON 대비 GP3가 가 장 높았지만, 대용량에서는 차이가 없었다. 6-G 측정 결과 소용량보 다 대용량으로 처리했을 때 GP3, GP1 모두 유의적으로 증가하였고, Total 함량은 소용량에서는 CON 대비 GP3가 1.7배 높아졌으며 대용 량에서는 약 1.3배 증가하였다. 결과적으로 생강 착즙박에 효소 전처 리 방법을 적용하는 것은 수용화 증진 및 유용성분 추출 함량을 증대시킬 수 있는 단순 공정의 유용한 기술로 사용될 수 있을 것이라고 판단된다. 또한 용량별 효소 처리 추출물의 기능성분 함량 비교결과 를 통해 생강 착즙박의 산업적 대량 생산을 위한 추출 공정 적용 가 능성을 확인하였으며, 이를 통해 부가가치 향상 및 기능성 소재로서 의 가능성을 제시하였다.

The polyphenol content of common buckwheat, Tartary buckwheat, buckwheat leaves, sea tangle, sea mustard ears, and fusiformis were analyzed. Among these, buckwheat leaves exhibited the highest polyphenol content. Hot water extraction followed by treatment with plantase enzyme increased the polyphenol yield by 56% for buckwheat leaves and by 34% for sea tangle. Cytotoxicity test performed on 3T3-L1 pre-adipocytes for seaweeds and buckwheat leaves revealed no significant cytotoxic effects compared to that of the control group at 1 mg/mL. Additionally, when examining the effects of buckwheat leaf and sea tangle extracts on the pre-adipocytes differentiation, into adipocytes confirmed that buckwheat leaf extract inhibited fat differentiation at 10 mg/mL and sea tangle at 0.1 mg/mL. Buckwheat/sea tangle enzyme food had 6.5 times higher amylase activity, 27 times higher protease activity, and more than twice as high dietary fiber, polyphenol, and DPPH scavenging ability as brown rice enzyme foods. Buckwheat/sea tangle enzyme-supplemented food contained more than 30% more phlorotannins and 7.8% more alginic acid than brown rice enzyme-supplemented food.

Green pesticides, derived from natural sources, have gained wider attention as an alternative approach to synthetic pesticides in managing polyphagous pests like Spodoptera litura. In this study, the methanolic flower extract of Nyctanthes arbor-tristis (Mx-Na-t) was subjected to chemical screening, and major peak area derivatives 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (3H-dp) and Tyrosol (Ty-ol) were identified. The toxicity against S. litura larvae of Mx-Na-t (at 500 ppm) was highest in third instars (96.4%), and for 3H-dp and Ty-ol (at 5 ppm) in II instars (76.5% and 81.4%, respectively). The growth and development of S. litura larvae and pupae were significantly reduced by all three extract and phytochemical treatments. Fecundity rates also declined with the treatments, from 1,020 eggs (control) to 540 eggs (Mx-Na-t), 741 eggs (3H-dp), and 721 eggs (Ty-ol). The extract and its active constituents decreased adult emergence and slowed total larval development in a dosedependent manner. The major gut enzymes of S. litura decreased in young larvae (II instar) exposed to Mx-Na-t, 3H-dp, and Ty-ol. Fourth instar midgut tissues were severely damaged by Mx-Na-t (250 ppm), 3H-dp, and Ty-ol (2.5 ppm) treatments, which induced structural damage to the epithelial cells and gut lumen. The earthworm Eisenia foetida was used to test for non-target toxicity. Crude Mx-Na-t at 500 ppm (13% and 3%) and 3H-dp (9.3% and 2.1%) and Ty-ol (10.2% and 1.5%) at 5 ppm produced lower mortality than the synthetic chemical cypermethrin at 1.0 ppm (27% and 18%) in filter paper and artificial soil assays, respectively. In addition, there was no significant change in earthworm weights under all three phytochemical treatments compared to controls. Additionally, the in-silico predictions of BeeTox and ProTox II indicated little or no toxicity toward honey bees and other nontargets associated with 3H-dp and Ty-ol. Overall these phyto-chemicals offer an effective pest management strategy.

본 실험에서는 외인성 효소 첨가제 및 혼합 세균 배양을 통한 고상발효(Solid-state fermentation, SSF)가 채종박(Rapeseed meal, RSM)의 체외건물소화율(In vitro dry matter digestibility, IVDMD) 및 단쇄지방산(Short chain fatty acid) 생성에 미치는 영향을 조사하기 위해 수행되었다. 외인성 효소 칵테일(첨가 및 미첨가) 및 RSM에 대한 SSF(발효 및 비발효)를 나타내는 2 x 2 요인 설계가 적용되었다. 3-step 돼지 소화율 모델을 적용하여 채종박의 건물 소화율을 분석하였으며, 72시간 대장발효 후 상층액을 수집하여 단쇄지방산 생성량을 분석한 후 칼로리 단위로 변환하여 가소화에너지 소화율을 분석하였다. 소장 (IVDMDh) 및 전장 (IVDMDt) 건물소화율에서는 고상발효된 채종박이 더 높게 나타났다 (각각 p < 0.01). 마찬가지로, 외인성 효소 첨가제 처리구에서 채종박의 소장 소화율(IVDMDh)이 증가하는 경향을 나타냈다(p = 0.06). Acetic acid 및 butyric acid의 생산은 대조구에 비해 고상발효 처리 시 유의하게 더 생산되었으며 (각각 p < 0.01), 이는 총 단쇄지방산의 생산 증가 경향을 나타냈다(p = 0.09). 에너지 소화율에서는 채종박의 고상발효 및 외인성 효소제 첨가가 유의적으로 높게 나타났다 ( p < 0.01). 그러므로 채종박의 고상발효 처리는 단백질 이용성을 비롯한 영양적 가치를 향상시키는데 효과적이라고 사료된다.

To produce super sweet corn sikhye, substituted for sweetener, the ratio of rice and super sweet corn was adjusted and processed with complex enzymes during saccharification, and their physicochemical and sensory properties were analyzed. The soluble solid content of the control and Corn-5 showed significantly high content at 13.50 °Brix, and the reducing sugar content of Corn-5 showed the highest content at 9.45%. The control showed the lowest free sugar content among all the experimental groups, excluding maltose content. In the enzyme-treated corn sikhye group, as the amount of super sweet corn increased, the content of sucrose decreased and the contents of glucose and fructose increased. The content of ascorbic acid and polyphenol compounds increased as the amount of super sweet corn increased. DPPH and ABTS radical scavenging abilities increased with increasing ratio of super sweet corn and enzyme treatment compared to the control. In the case of sensory evaluation, Corn-3, which substituted 30% of super sweet corn for rice and treated with enzymes, showed higher evaluations in taste, sweetness, and overall preference than those of the control.

To improve usability of super sweet corn, extracts were prepared with hydrolytic enzyme and changes in physicochemical and antioxidant properties were analyzed. Soluble solids and reducing sugars contents were higher in all enzyme treatment groups than in the control. When enzyme treatment time increased, contents of soluble solids and reducing sugars were also increased. There was no significant difference in lightness between treatment groups, with redness showing the highest value in the control and yellowness showing the highest value in the invertase treatment group. Free sugar content in the control was the lowest. However free sugar content in the enzyme combination treatment group was increased by more than four times compared to that in the control. Contents of ascorbic acid, flavonoids and polyphenols were higher in the enzyme treatment group than in the control. In particular, the enzyme combination treatment group showed the highest content. DPPH and ABTS radical scavenging abilities were significantly higher in all enzyme treatment groups than in the control. Radical scavenging abilities of cellulase treatment group and enzyme combination treatment group showed high activity. The activity increased when enzyme treatment time increased. The combined enzyme treatment method for super sweet corn was suitable for food processing.

This study investigated the effects of pectinase treatment and skin contact time on the quality characteristics of Dae-hong peach wine. Wine was produced with variations in enzyme treatment and skin contact time (1 hour, 2 hours, 1 day, 2 days, and until the completion of fermentation). Enzyme treatment increased the production yield by 6%, as well as ethanol and redness levels, compared to the non-treated control. Volatile components were higher when the skin contact time was 2 hours or 1 day. Results were compared according to enzyme treatment and skin contact time and found to be influenced by methanol and 3-methyl-1-butanol. Enzyme treatment effectively enhanced yields and volatile compound contents. However, skin contact should be concluded a day before 1 day to ensure compliance with methanol legislative requirements. Therefore, our findings show that enzymatic treatment with shorter skin contact time preserves the distinctive characteristics of Dae-hong peaches and ensures the production of safe and flavorful wine.

효소 고정화 막 생물반응기(EMBRs)는 폐수 내의 염료를 처리하는 새로운 방법입니다. 이 분야는 효소의 효능과 환경에 대한 높은 저항성 때문에 많은 양의 연구가 진행되었습니다. 효소 자체와 해당 효소의 구조를 모두 포함하는 다양한 방법이 EMBR에 접근할 수 있습니다. 생물반응기 자체는 염료 제거의 필요에 맞게 변형될 수 있습니다. 효소적 생물반응기 부터 산화 그래핀 또는 탄소 나노튜브와 같은 나노구조를 사용하는 것까지 다양합니다. 또한 TiO2와 같은 나노입자는 EMBR 을 더욱 향상시키기 위해 사용될 수 있습니다. 폴리머 기반의 막 지지 구조는 또한 효능 증가의 문제에 접근하는 다양한 방 법을 포함합니다. 본 바와 같이, 지난 수십 년 동안 EMBR을 사용하는 이 문제에 대한 다양한 접근법이 수행되었습니다. 이 검토는 방법론을 요약하고 EMBR에 대한 다양한 개선 사항을 설명하는 것을 목표로 합니다.

The enzyme digestion of foods is known to have certain advantages, such as enhancing health improvement functions of functional foods. In this study, we investigated whether the memory enhancement effects of HongJam could be enhanced by enzymatic digestions. We found that enzyme digested HJ had more enhanced functionality than undigested HJ. We also found the molecular basis of memory enhancement by performing various biochemical and molecular biological experiments. (Grant No. PJ016908032023)

본 연구의 목적은 남자 대학 엘리트 조정선수의 2000 m 로잉 에르고미터 수행 후 저온침수 처 치를 통해 혈중 젖산, LDH, MDA 및 SOD의 변화에 긍정적인 영향을 주어 피로 회복에 미치는 영향을 구명하는 데 있다. 이에 남자 대학 엘리트 조정선수 10명을 대상으로 고강도 로잉 에르고미터 2,000 m 수 행 후 비 처치와 저온 침수 처치의 효과를 비교하였다. 측정 변인들에 대한 결과를 검증하기 위해 처치 및 시기 간 상호작용 효과를 분석하기 위해 ANOVA를 실시하였고 각 항목별 유의수준 .05로 설정하여 다음 의 결과를 도출하였다. 젖산은 시기 간 주효과가 나타났고(p<.001) 그룹 내 시기별 차이가 났다(p<.001). 또한, LDH는 그룹 내 시기별 차이가 나타났다(p<.05). MDA는 그룹×시기 간 상호작용 효과가 나타났고 (p<.05), 그룹 간(p<.05), 시기 간(p<.001) 주효과가 나타났다. SOD는 그룹 간, 시기 주효과가 나타났고 (p<.05) 회복 30분 후 그룹 간 차이가 나타났다(p<.05). 이를 종합해 볼 때, 본 연구에서 실시한 저온침수처치가 조정 선수의 혈중 피로 물질, 활성산소 및 항산화 효소에 유의한 효과를 나타냈다. 따라서 운동선수 의 고강도 훈련 후 저온침수를 적극 활용할 것을 권장한다.

To understand antioxidant enzyme response of two contrasting Arundinella hirta ecotypes to drought stress, drought-tolerant Youngduk and drought-sensitive Jinju-1, were comparatively analyzed changes in the enzymatic activities of peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR). Two ecotypes, drought-tolerant Youngduk and drought-sensitive Jinju-1 were subjected to drought stress by withholding water for 12 days. ROS accumulation level and electrolytic leakage were significantly increased in both A. hirta ecotypes by drought stress treatment but less in Youngduk than Jinju-1. The RWC significantly decreased in both the drought stress-treated ecotypes as compared to control, but less in Youngduk than Jinju-1. Soluble sugar and protein content were increased more in drought stress-treated Youngduk as compared to Jinju-1. The activities of antioxidant enzymes such as SOD, CAT, POD, APX, and GR increased significantly in both the drought stress-treated ecotypes Youngduk and Jinju-1 as compared to control. The increase in antioxidant enzyme activity level was more prominent in drought stress-treated Youngduk as compared to Jinju-1. Taken together, these results suggest that Youngduk was more tolerant to drought stress than Jinju-1, and seem to indicate that tolerance of A. hirta to drought stress is associated with increased activity of antioxidant enzymes.

This study selected the optimal conditions for enzyme treatment that can promote the extraction of functional components from Salvia plebeia (SP) and confirmed solubilization characteristics, functional component contents, and applicability evaluation in pilot-scale, antioxidative, and anti-inflammatory activity. The optimal conditions of pectinase, 1%, and 2 h (P) were determined through the enzyme treatment of SP. The water-soluble index and total polyphenol content were the highest in P compared to 100oC reflux condition for 2 h (CON). Component analysis performed using liquid chromatography-mass spectrometry revealed that flavonoid contents were increased 6.6-9.5 times in the P compared to CON. There was no difference in component content when comparing the measurement lab-scale and pilot scale. CON and P indicated cytoprotective effect against H2O2-induced RAW 264.7 cells. CON and P pre-treatment also reduced the production of nitric oxide and proinflammatory cytokines in LPS-induced RAW 264.7 cells. Furthermore, P showed higher antioxidant and anti-inflammation activities than CON. These results suggested that P has a higher concentration of bioactive compounds through enzyme treatment than that obtained from CON. Thus, it can be used as a primary material for industrial utilization, such as developing functional food materials using SP.