RNA interference (RNAi) technology based on feeding double-stranded RNA (dsRNA) has been employed for the control of insect pests. In general, strong lethal effects have been observed when feeding RNAi is applied to chewing insects. However, the efficacy of feeding RNAi for sap-sucking insects has not been reported to be limited most likely due to the reduced rate of dsRNA translocation into the plant sap. In this experiment, therefore, we tested whether the long-hairpin RNA (lhRNA) structure, which mimics the viroid, can improve its translocation within plant tissues, thereby increasing lethality of target gene, when compared with dsRNA structure. Either lhRNA of dsRNA structure (75 ng/ul) of vacuolar ATP synthase subunit A (V-ATPase) gene was delivered via rice seedling to Nilaparvata lugens, which is one of the major sucking insects on rice, and mortality was measured until 60 h post-treatment. Treatment of the lhRNA and dsRNA of V-ATPase gene caused increased mortality over time compared with eGFP-treated control, reaching the maximum level at 48 h post-treatment, and the mortality was significantly higher in lhRNA treatment than in dsRNA treatment. Gene silencing of target gene was confirmed at 24 h and 48 h post-treatment. In summary, treatment of lhRNA resulted in significantly higher mortalities than that of dsRNA, suggesting that delivery of lhRNA has an apparent advantage over dsRNA in exerting RNAi-induced lethality.

In the case of one standard-mum polyvinyl house, the density of F. occidentalis reached its peak in early June and decreased after the chemical control, which was carried out periodically. In the case of another standard-mum polyvinyl house, the density of F. occidentalis increased continuously in spite of the periodical chemical control after planting. In both polyvinyl houses, the density of F. occidentalis began to increase mid-May. The numbers of F. occidentalis collected on a tray with wet paper by heating the flowers of pink, white, and yellow Chrysanthemum standard mums were 18.4, 56.6, and 52.6 after flowering, but in the case of the leaves, the numbers were 7.8, 16.6, and 15.4. Also, the numbers of F. occidentalis collected on a tray with wet paper by heating the buds of pink, white, and yellow Chrysanthemum standard mums were 15.2, 45.8, and 41.6, but in the case of the leaves, the numbers were 2, 8.8 and 3.4. In the Y-tube olfactometer test, the frequency of F. occidentalis’ visits to one side arm of the Y-tube olfactometer was higher in the odor cue of the white flower than of the yellow, red, and violet flowers, but in the other combinations without white flowers, the frequency was higher in the odor cue of the violet and red flowers than of the yellow flowers. In the case of the four-choice olfactometer test, in the same visual cues as the odor cues of the pot mum flowers, the frequency of F. occidentalis was higher in the yellow flower than in the other flowers (white, red, and violet) in all the observation times (10, 15, and 20 minutes). In the case of the visual cues with disturbed odor cues, F. occidentalis preferred the yellow color to the other colors in all the observation times (10, 20, and 40 minutes). F. occidentalis preferred the white color to the other colors when the same odor cues as the flower color were provided, but the odor cue of the yellow flower was provided only without the color cue.

Spatial association of entomopathogenic and free-living nematode populations were analyzed at the landscape scale. Free-living nematodes were classified into functional guilds based on their trophic level (i.e. bacterivores, carnivores, fungivores, and omnivores) and life history characteristics (i.e. r-selected colonizing versus K-selected persisting species) in the soil ecosystem. Differences in life history traits were hypothesized to result in different spatial associations of populations. Spatial aggregation indices were calculated for each nematode group. Spatial associations were analyzed and compared for entomopathogenic nematodes (EPN) and each trophic/life history group of free-living nematodes based on spatial analysis of distance indices (SADIE). Spatial aggregation indices (Ia) revealed that taxa with more K-selected persisting life history were less aggregated in their habitat, whereas more r-selected colonizer life history types showed more aggregation. Spatial aggregation index of EPN populations was similar to that of relatively r-selected colonizer type free-living nematodes, which share several life history traits including high reproductive rates and insect phoresy. There were substantial spatial associations between EPN and r-selected colonizer life history free-living nematode taxa.

We investigated the molecular and kinetic properties of two acetylcholinesterases (AmAChE1 and AmAChE2) from the Western honey bee, Apis mellifera. Western blot analysis revealed that AmAChE2 has most of catalytic activity rather than AmAChE1, further suggesting that AmAChE2 is responsible for synaptic transmission in A. mellifera, in contrast to most other insects. AmAChE2 was predominately expressed in the ganglia and head containing the central nervous system (CNS), while AmAChE1 was abundantly observed not only in the CNS but also in the peripheral nervous system/non-neuronal tissues. Both AmAChEs exist as homodimers; the monomers are covalently connected via a disulfide bond under native conditions. However, AmAChE2 was associated with the cell membrane via the glycophosphatidylinositol anchor, while AmAChE1 was present as a soluble form. The two AmAChEs were functionally expressed with a baculovirus system. Kinetic analysis revealed that AmAChE2 has approximately 2,500-fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than AmAChE1, supporting the synaptic function of AmAChE2. In addition, AmAChE2 likely serves as the main target of the organophosphate (OP) and carbamate (CB) insecticides as judged by the lower IC50 values against AmAChE2 than against AmAChE1. When OP and CB insecticides were pre-incubated with a mixture of AmAChE1 and AmAChE2, asignificant reduction in the inhibition of AmAChE2 was observed, suggesting a protective role of AmAChE1 against xenobiotics. Taken together, based on their tissue distribution pattern, molecular and kinetic properties, AmAChE2 plays a major role in synaptic transmission, while AmAChE1 has non-neuronal functions, including chemical defense.

The Herschel Space Observatory is the European Space Agency's state of the art infrared space telescope launched into space on 14 May 2009, covering the wavelength range from 70-700 microns with 3 instruments SPIRE, PACS and HIFI. Large area surveys are being carried out by Herschel in the AKARI legacy fields at the North and South Ecliptic Poles and the AKARI All-Sky Survey provides additional synergy with the largest survey with Herschel, H-ATLAS, covering more than 500 square degrees. This paper reports on some of the early results of these synergies between Herschel and AKARI including the first comparison of the AKARI All-Sky Survey number counts with the deeper Herschel surveys.

This paper presents Hα emission line detections for four galaxies at z > 3.5 made with AKARI as part of the FUHYU mission program. These are the highest-redshift Hα detections to date in star-forming galaxies. AKARI's unique near-infrared spectroscopic capability has made these detections possible. For two of these galaxies, this represents the first evidence of their redshifts and confirms their physical association with a companion radio galaxy. The star formation rates (SFRs) estimated from the Hα lines under-predict the SFRs estimated from their far-infrared luminosities by a factor of ~ 2 - 3. We have also detected broad Hα components in the two radio galaxies which indicate the presence of quasars.

We present the 18μm luminosity function (LF) of galaxies at 0.006 < z < 0.8 (the average redshift is ~ 0.04) using the AKARI mid-infrared All-Sky Survey catalogue. We have selected 243 galaxies at 18μm from the Sloan Digital Sky Survey (SDSS) spectroscopic region. These galaxies then have been classified into five types; Seyfert 1 galaxies (Sy1, including quasars), Seyfert 2 galaxies (Sy2), low ionization narrow emission line galaxies (LINER), galaxies that are likely to contain both star formation and Active Galactic Nuclei (AGN) activities (composites), and star forming galaxies (SF) using optical emission lines such as the line width of H α or the emission line ratios of [OIII]/ Hβ and [NII]/ Hα . As a result of constructing the LF of Sy1 and Sy2, we found the following results; (i) the number density ratio of Sy2 to Sy1 is 1.64±0.37 , larger than the results obtained from optical LF and (ii) the fraction of Sy2 in the entire AGN population may decrease with 18μm luminosity. These results suggest that most of the AGNs in the local universe are obscured by dust and the torus structure probably depends on the mid-infrared luminosity.

ANIR (Atacama Near InfraRed camera) is a near infrared camera for the University of Tokyo Atacama 1m telescope, installed at the summit of Co. Chajnantor (5,640 m altitude) in northern Chile. The high altitude and extremely low water vapor (PWV = 0.5 mm) of the site enable us to perform observation of hydrogen Paα emission line at 1.8751 μm . Since its first light observation in June 2009, we have been carrying out a Paα narrow-band imaging survey of nearby luminous infrared galaxies (LIRGs), and have obtained Paα for 38 nearby LIRGs listed in AKARI/FIS-PSC at the velocity of recession between 2,800 km/s and 8,100 km/s. LIRGs are affected by a large amount of dust extinction ( AV~ 3 mag), produced by their active star formation activities. Because Paα is the strongest hydrogen recombination line in the infrared wavelength ranges, it is a good and direct tracer of dust-enshrouded star forming regions, and enables us to probe the star formation activities in LIRGs. We find that LIRGs have two star-forming modes. The origin of the two modes probably come from differences between merging stage and/or star-forming process.

A study on the indigenous diatoms was carried out at 103 sites during August 2008 to April 2011 from the marine and the brackish waters in Korea. A fine structure of small-sized diatoms was examined using a light and scanning electron microscopy. Sixty species of diatoms were newly identified and composed of 3 class, 6 subclass, 16 order, 21 family and 39 genus. The detailed nomenclatures, references, photographs and distributions were here reported. Of 60 species, the most frequent species was Tryblionella coarctata appearing 24 times, and 22 times followed by Thalassiosira lacustris, Cocconeis stauroneiformis was 20 times. In the level of genus, Chaetoceros, Parlibells and Thalassiosira were included 4 species, Actinocyclus, Minidiscus and Licmophora were included 3 species. This study will provide the knowledge of the diversity level of diatom in Korea, the knowledge is important on the diatoms for further studies.

Understanding the birth and evolution of galaxies, and the history of star formation in them, is one of the most important problems in astronomy. Using the data from the AKARI IRC survey of the Large Magellanic Cloud at 3.2, 7, 11, 15, and 24 μm , we have constructed a multi-wavelength catalog containing data from the cross-correlation with a number of other databases at different wavelengths. We present the first approach with a Support Vector Machine (SVM)-based method to separate different classes of stars in LMC in the color-color and color-magnitude diagrams.

Sphingosine-1-phosphate (S1P) has a many function involved proliferation, differentiation and survival of many cells. In this study, to investigate whether S1P improve the developmental competence of porcine embryos, 50 nM of S1P were supplemented during in vitro maturation (with EGF or without EGF) medium and/or in vitro culture (IVC) medium. Addition of S1P was significantly increased the rate of oocytes reaching metaphase II (MII) compared to the control (83.5 vs. 64.1%) in without EGF medium, but not with EGF medium (89.5 vs. 84.6%). When treated with 1 μM of N1N-dimethylsphingosine (DMS), a sphingosine kinase inhibitor which is blocked endogenous generation of S1P, the meiotic progression rates to MII stage (without EGF: 45.2 and with EGF: 66.7%) were significantly decreased and degeneration rates (without EGF: 51.2 and with EGF: 30.1%) were increased in both medium compared to control group during IVM periods. Also, the rates of blastocyst formation was significantly increased in the S1P treated group compared to control group (29.0 vs. 19.2%) of EGF supplemented medium, whereas there were no effect in the EGF free medium (9.0 vs. 10.5%). After 12 h IVM, the phosphorylation of ERK1 and ERK2, which is major signaling pathway of MAP kinase, were increased in the S1P group than that of control or DMS group. When supplemented of S1P during IVC, the rates of blastocyst formation and total cell number (30.2% and 40.6) were significantly increased in S1P-treated group compared with control (20.1% and 32.5), DMS (12.3% and 25.1), and S1P plus DMS group (24.7% and 33.6). The percentage of apoptosis nuclei in the S1P group was significantly decreased than other groups. Also, the rates of blastocyst formation (26.7 vs. 14%) and total cell number (42.8 vs. 32.5) were significantly increased in the S1P group than those of control group when S1P added during the entire IVM and IVC periods. Taken together, our results indicate that S1P supplementation in IVM and/or IVC medium affects beneficial effect of meiotic maturation and subsequent developmental competence of porcine embryos.

We have created new catalogues of AKARI/IRC 2 − 24 μm North Ecliptic Pole Deep survey through new methods of image analysis. In the new catalogues the number of false detection decreased by a factor of 10 and the number of objects detected in multiple bands increased by more than 1,500 compared to the previous work. In this proceedings the new methods of image analysis and the performance of the new catalogues are described.

We present a photometric catalog of infrared (IR) sources based on the North Ecliptic Pole Wide field (NEP-Wide) survey of AKARI, which covered a 5.4 deg 2 circular area centered on NEP. The catalog contains about 115,000 sources detected at the 9 IRC filter bands, comprehensively covering a wavelength range from 2 to 24 μm . This is a band-merged catalog including all of the photometry results from the supplementary optical data as well as the IRC bands. To validate a source at a given IRC band, we searched for counterparts in the other bands. The band-merging was done based on this cross-matching of the sources among the filter bands. The NIR sources without any counterpart in any other bands are finally excluded to avoid false objects.

Poly-L-lactic acid (PLLA), PLLA/hydroxyapatite (HA), PLLA/multiwalled carbon nanotubes (MWNTs)/HA, PLLA/trifluoroethanol (TFE), PLLA/gelatin, and carbon nanofibers (CNFs)/β-tricalcium phosphate (β-TCP) composite membranes (scaffolds) were fabricated by electrospinning and their morphologies, and mechanical properties were characterized for use in bone tissue regeneration/guided tissue regeneration. MWNTs and HA nanoparticles were well distributed in the membranes and the degradation characteristics were improved. PLLA/MWNTs/HA membranes enhanced the adhesion and proliferation of periodontal ligament cells (PDLCs) by 30% and inhibited the adhesion of gingival epithelial cells by 30%. Osteoblast-like MG-63 cells on the randomly fiber oriented PLLA/TEF membrane showed irregular forms, while the cells exhibited shuttle-like shapes on the parallel fiber oriented membrane. Classical supersaturated simulated body fluids were modified by CO2 bubbling and applied to promote the biomineralization of the PLLA/gelatin membrane; this resulted in predictions of bone bonding bioactivity of the substrates. The β-TCP membranes exhibit good biocompatibility, have an effect on PDLC growth comparable to that of pure CNF membrane, and can be applied as scaffolds for bone tissue regeneration.

The North Ecliptic Pole (NEP) Wide survey covered about 5.4 deg 2 , a nearly circular area centered on the NEP, using nine passbands of InfraRed Camera (IRC). We present the photometric properties of the data sets, and the nature of the sources detected in this field. The number of detected sources varied according to the filter band: with about 109,000 sources in the NIR, about 20,000 sources in the MIR-S, and about 16,000 sources seen in the MIR-L channel. The 5σ detection limits are about 21 mag in the NIR and 19.5 - 18.5 mag in the MIR bands in terms of the AB magnitude. 50% completeness levels are about 19.8 mag at 3 μm , 18.6 mag at 9 μm , and 18 mag at 18 μm band (in AB magnitude), respectively. In order to validate the detected sources, all of them are confirmed by matching tests with those in other bands. The 'star-like' sources, defined by the high stellarity and magnitude cut from the optical ancillary data, appear statistically to have a high probability of being stars. The nature of the various types of extragalactic sources in this field are discussed using the color-color diagrams of the NIR and MIR bands with the redshift tracks of galaxies providing useful guidelines.

본 연구는 온도 증가에 따른 압축을 받는 H형 강재의 플랜지와 웨브의 국부 및 전체좌굴응력 내화해석 프로그램 개발과 플랜지와 웨브가 항복파괴전에 국부좌굴이 일어나지 않을 한계 판폭두께비의 상관값을 구하는 프로그램을 개발하는 것이다. 고온에서의 강재의 응력-변형도 관계식은 EC3:Part 1.2를 근거로 하였으며, 비교, 검토를 위하여 영국 BS5950의 강재를 대상으로 온도 증가에 따른 압축을 받는 강재의 플랜지와 웨브의 파괴온도와 하중을 본 연구의 내화해석 프로그램으로 예측하였다. 본 연구는 좌굴 및 항복에 대한 내화해석 프로그램을 개발하는 것을 목적으로 하고 적용 예를 통하여 좌굴 및 한계 판폭두께비를 분석하고 개발 프로그램의 타당성을 검토하였다.

The bio-organic thin film transistor (BiOTFT) with the DNA and DNA-surfactant complex as a dielectric layer shows memory function. In order to investigate the effect of surfactant structure on the OTFT memory device performance, different kinds of surfactant were introduced. The octadecyltrimethylammonium chloride (OTMA), ctyltrimethylammonium chloride (CTMA), or lauroylcholine chloride (Lau) as cationic surfactant as mixed with DNA to prepare the DNA complex through the electrostatic interaction. In addition, the different molecular weight DNA also has been studied to analyze the effect of DNA chain length on the performance of the physical property. Many kinds of methods including UV-vis, Circular dichiroism (CD), I-V characteristic and atomic force microscope (AFM) have been applied to analyze the property of DNA complex. In conclusion, all of DNA complex with CTMA, OTMA and Lau revealed to work as the bio-organic thin film transistor memory, and the device fabricated by Lau has the highest ON current and showed better device performance.

A chemical process involves polymerization within microspheres, whereas a physical process involves the dispersion of polymer in a nonsolvent. Nano-sized monodisperse microspheres are usually prepared by chemical processes such as water-based emulsions, seed suspension polymerization, nonaqueous dispersion polymerization, and precipitation polymerizations. Polymerization was performed in a four-necked, separate-type flask equipped with a stirrer, a condenser, a nitrogen inlet, and a rubber stopper for adding the initiator with a syringe. Nitrogen was bubbled through the mixture of reagents for 1 hr. before elevating the temperature. Functional silane (3-mercaptopropyl)trimethoxysilane (MPTMS) was used for the modification of silica nanoparticles and the self-assembled monolayers obtained were characterized by X-ray photoelectron spectroscopy (XPS), laser scattering system (LSS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), elemental analysis (EA), and thermogravimetric analysis (TGA). In addition, polymer microspheres were polymerized by radical polymerization of γ-mercaptopropyl modified silica nanoparticles (MPSN) and acrylamide monomer via precipitation polymerization; then, their characteristics were investigated. From the elemental analysis results, it can be concluded that the conversion rate of acrylamide monomer was 93% and that polyacrylamide grafted to MPSN nanospheres via the radical precipitation polymerization with AAm in ethanol solvent. The microspheres were successfully polymerized by the 'graft from' method.

Peri-implantitis (PI) is bacteria-induced inflammatory condition which affects the alveolar bone and soft tissue around implants and may result in the loss of supporting bone. Attenuation of the P. gingivalis lipopolysaccharide (LPS)-induced inflammatory response can be a new therapeutic approach in the treatment of PI. This study was conducted to evaluate the anti-inflammatory effect of 635-nm light-emitting diode (LED) irradiation over MG63 osteoblast-like cell. Scratch was made on MG63 cells with or without LPS, then 635-nm irradiated. The expression of the cyclooxygenase-2 (COX-2) proteins was evaluated with western blot. The production of the prostaglandin E2 (PGE2) and expression of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) was measured with enzyme-linked immunoassay, and the cytokine profile was evaluated with the human inflammation antibody array. Wound closure effect presented in the cells treated with LPS was observed more significantly in the cells with 635-nm irradiation than the cells without irradiation. The 635-nm irradiaiton reduced LPS-induced expression of the COX-2 and production of the PGE2. Also, 635-nm irradiation affect the expression of RANKL, OPG, and proinflammatory cytokines. These results indicate that 635-nm irradiation could reduce the alveolar bone resorption induced by LPS stimulation through the inhibition of COX-2 expression and PGE2 production, the suppression of proinflammatory cytokine, and the modulation of RANKL/OPG balance in MG63 cells.