검색결과

검색조건
좁혀보기
검색필터
결과 내 재검색

간행물

    분야

      발행연도

      -

        검색결과 1,257

        621.
        2008.05 구독 인증기관·개인회원 무료
        The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion improved the secretion and antibacterial activity of recombinant nuecin proteins. Thus, bPDI gene fusion is a useful addition to the BEVS for the large-scale production of bioactive recombinant proteins.
        623.
        2008.04 구독 인증기관·개인회원 무료
        624.
        2008.02 구독 인증기관 무료, 개인회원 유료
        To maintain optimal performance in a floor heating system, the elements of system must be consisted of the adequate structure in order that the heat flow is efficiently transmitted from the floor structure to indoor space. The aims of this study is to propose the optimal structure type and present applicable design data of a floor heating system. The design data suggested in this study can be applied to cooperate energy efficiency design as well as comfortable indoor thermal environment in the early design stage.
        4,000원
        625.
        2008.02 구독 인증기관 무료, 개인회원 유료
        This study is Korean traditional heating system, named 'AZABANG'. Ondol characterized by" Azabang "is dated back to 119 AD and it has approximately 2000 years history. Among various kinds of traditional radiant floor heating system, Ondol is the most reasonable and scientific one. This type of imaginative Ondol which have superior ability to keep temperature during a long period, roughly I 00 days could be attributable to the special structural pattern . The Ondol characterized by ''Azabang" represents extraordinary creativeness originated from Korean traditional culture and at the same time, it is the significant evidence that those missing historical rings, in some degree, have been connected. Therefore we well do our best to apply the scientific and reasonable concept into the real world.
        4,000원
        626.
        2007.12 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        We calculate the evolution of multiple supernova (SN) explosions inside a pre-exiting bubble blown up by winds from massive stars, using one-dimensional hydrodynamic simulations including radiative cooling and thermal conduction effects. First, the development of the wind bubble driven by collective winds from multiple stars during the main sequence is calculated. Then multiple SN explosion is loaded at the center of the bubble and the evolution of the SN remnant is followed for 106 years. We find the size and mass of the SN-driven shell depend on the structure of the pre-existing wind bubble as well as the total SN explosion energy. Most of the explosion energy is lost via radiative cooling, while about 10% remains as kinetic energy and less than 10% as thermal energy of the expanding bubble shell. Thus the photoionization and heating by diffuse radiation emitted by the shock heated gas is the most dominant form of SN feedback into the surrounding interstellar medium.
        3,000원
        628.
        2007.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In the present study, electrospun PAN precursor webs and the stabilized and carbonized nanofiber webs processed under different heat-treatment conditions were characterized by means of weight loss measurement, elemental analysis, scanning electron microscopy (SEM), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), thermogravimentric analysis (TGA), and X-ray diffraction (XRD) analysis. The result indicated that stabilization and carbonization processes with different temperatures and heating rates significantly influenced the chemical and morphological characteristics as well as the thermal properties of the stabilized and then subsequently carbonized nanofiber webs from PAN precursor webs. It was noted that the filament diameter and the carbon content of a carbonized nanofiber web as well as its weight change may be effectively monitored by controlling both stabilization and carbonization processes.
        4,000원
        630.
        2007.10 KCI 등재 구독 인증기관·개인회원 무료
        3BK21 program for Veterinary Science, College of Veterinary Medicine‘ Seoul National University. Seoul. Korea Human Co1'd blood has been used for the alternatives of bone marrow transplantation for more 10 years. Recently Mesenchymal s tem cell s , ES-like cells and endothelial stem cells has been successfuly isolated from huam co1'd blood Presentl y. it has been reported that a bout 70 incurable ans tractible di sease was possibly cured by umbili cal cord blood-deri ved s tem cells in the clinit;al test s‘ However‘ isolation and expansion of s tem cells from human umbilical cord blood(UCB) have been very difficult and an obstrucle for the clinical use This study showed that effi cient s iolat iona and expans ion of mesenchymal stem cells from UCB Full term UCB samples were obtained from the umbi lical vein after vaginal deli ve ry with the informed consent 0 1' the mothe1' approved by Borame Hospital Institutional Review Broad (IRB). And a lso. t his work was also a pproved by Seoul National University IRB. Recently, we isolated a population of s tem cells from human corcl bloocl (UCB)‘ which expressed embryo stage specific maker. SSEA-4. ancl the multi-potential stem cell marker‘ 。c t4 And we have sucessfully developed culture methods to expand ancl subculture these cells up to 1.000 billion from one single clone. Subsequently. we were a ble to transclifferente theses stem cells into insulin- producing is let- like structures. which co-express in sulin andC-pepticle, adipocyte, neuron‘ bone and cartilage. In acldition. the isola tion rate of MSC from UCB is about 70 % from the cord blood units. This isolation rate were not affected by maternal ages. the sex of baby, isolation time from the deli very. for example. 12 hrs. 24 hrs ‘ even 48 hrs from delivery Taken together. these findings might have a s ignificant potential to aclvance human UCB clerivecl stem- cell -basecl ther apeutics fOI' clinical use in near future
        637.
        2007.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        L2 speakers often encounter difficulties caused by a dual problem both with vocabulary and structure in delivering his or her intended message. This qualitative study reports on how the dual problem involving both lexical and syntactic problems can be managed by employing CSs. The participants were 12 Korean students studying in a university in the USA, whose language proficiency varied from limited to high advanced. For the data, the participants’ natural conversations in various settings were recorded and stimulated recalls were conducted on the recorded conversations. The study presents various CSs for managing dual lexical-syntactic problems (DL-SCSs) identified from an inductive analysis of the data. Based on a taxonomic analysis of the data, this study presents a taxonomy in which various DL-SCSs are organized in multiple levels. In this paper, examples illustrating how participants employed each DL-SCS are also provided. These examples show that L2 speakers employing appropriate DL-SCSs are likely to communicate their intended meaning despite massive dual problems. Based on the findings, this study suggests that L2 speakers should be encouraged to use DL-SCSs.
        6,000원
        638.
        2007.09 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        6,100원
        639.
        2007.09 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        7,800원
        640.
        2007.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study investigated how task involvement should be practiced to fulfill the theoretical assumptions of Task-Based Instruction (TBI). As the task involvement is to be understood as an indispensable component of TBI, how a high degree of task involvement can be secured and exerted in a most effective way was examined. Having analyzed the data obtained from questionnaire, interviews, reflection papers and proficiency tests, the study indicated that customized and timely feedback and comments on the learners’ performances as well as learners’ sense of responsibility were critical if the task involvement leads to desirable learning outcomes. The study also showed that consciousness-raising (CR) task originated from an authentic task was effective enough to help learners acquire linguistic knowledge in a contextual situation. It made suggestions for how to promote task involvement to better implement TBI, through which the learners can improve language proficiency fully taking advantage of their educational and cognitive experiences.
        8,400원