Through the research of tracheophytes type around Mt. Daedun, we are aiming at figuring out the habitat condition of useful resource plants by the classification of vegetation units and communities using the Z-M phyto-sociological method in the basis of the traits of species composition and by the analysis of vegetation and environment by way of coincidence method. Tracheophytes in our research sites was classifid into 117 family, 475 genus 797, species 102 variety,18 breed, in total to 917 kinds. The forest around Mt. Daedun district was classified into 6 communities, 12 groups, 2 sub-groups according to feature species group, differential species group and differential. Judging the result from the examination of community classification factors by the coincidence method, vegetation unit was decided by altitude and topography.
Sixteen isolates showing relatively strong antagonicity against the ginger rhizome rot pathogen, Pythium zingiberum, were selected among the 155 isolates from ginger rhizome surfaces and rhizospheres of ginger cultivation fields in Wanju, Chonbuk. The isolate, 'HB 26-5'showing the strongest antagonicity was finally selected by testing duration of inhibition effect and pathogenicity to ginger. The isolated antagonistic microorganism, 'HB 26-5' was rod shape, gram positive and formed endospore. The isolate produced acids utilizing glucose, arabinose, xylose and mannitol, and acetoin at VP test, and grew anaerobically. Temperature range for growth was from 10 to 40℃ . Reaction to catalase and gelatin, hydrolysis were positive, and casein hydrolysis and indol production were negative. Based on the mycological characters and the fatty acid composition, it was identified as Bacillus polymyxa. The pathogenicity test of isolated Bacillus polymyxa 'HB 26-5'on 22 crop cultivars resulted that only the lettuce was influenced in germination, and the others were not affected.
Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.
An isolate of barley yellow mosaic virus(BaYMV-HN) obtained from Haenam, Korea was compared with two BaYMV strains. BaYMV-Ⅱ-1 from Japan and BaYMV-G from Germany. The sequence of the 3'-terminal 3817nucleotides[excluding the poly (A) tail] of RNA 1 of BaYMV-HN was determined to start within a long open reading frame coding for a part of the NIa-VPg polymerase(26 amino acids). NIa-Pro polymerase (343 amino acids), NIb polymerase(528 amino acids) and the entire capsid protein(297 amino acids), which is followed by a noncoding region(NCR) of 235 nucelotides. In the partial ORFs, BaYMV-HN shows higher sequence homology with BaYMV-Ⅱ-1(99.5%) than BaYMV-G(92.7%). The 3' non-coding regions of BaYMV-HN(235nt) shows higher nucleotide sequence homology with BaYMV-G(235nt)(99.6%) than BaYMV-Ⅱ-1(231nt)(97.0%). The 3' NIa-Pro protein sequence of BaYMV-HN shows higher amino acid sequence homology with BaYMV-Ⅱ-1(95.0%) than BaYMV-G(93.6%), but, NIb protein sequence of BaYMV-HN shows same all amino acid sequence. The capsid protein sequence of BaYMV-HN(297aa) shows same with BaYMV-Ⅱ-1, and shows higher nucleotide sequence homology with BaYMV-UK (from United Kingdom)(97.3%) than BaYMV-G(96.9%) and G2(96.9%). Difference of capsid protein amino acid were 0-9 between the Japan, United Kingdom and Germany and were 2-6 between all Korean isolates. Many of the amino acid differences are located in the N-terminal regions of the capsid proteins from 1 to 74 amino acid positions.
Carotenoid compounds in embryos of wild-type(WT) and viviparous mutants of maize(Zea mays L.) were analyzed using high performance liquid ehromatography (HPLC) with a photodiode array detector. Zeaxanthin accumulates in WT embryos as the major carotenoid. Phytoene accumulates in vp2 and vp5. Phytofluene in w3 and ζ-carotene in the vp9 mutant embryos. This indicates that the vp2 and vp5 mutants impair phytoene desaturase from 15-cis-phytoene to 15-cis-phytofluene. The w3 mutant has neither an isomerase from 15-cis-phytofluene to all-trans-phytofuene nor phytofluene desaturase from phytofluene to ζ-carotene. The vp9 mutant does not have the ζ-carotene desaturase from ζ-carotene to lycopene. Our analysis shows that the terminal carotenoid. γ-carotene(β,ψ-carotene), accumulates in the vp7 mutant embryos. The ε-carotene(ε,ε-carotene), a product of δ-carotene(ε,ψ-carotene) in some plants, however, has not been found in maize embryos. The vp7 mutant impairs a cyclization step from γ-carotene to both β-carotene and α-carotene. We suggest that monocyclic γ-carotene is the sole precursor of both bicyclic β-carotene(β,β-carotene) and α-carotene(β,ε-carotene) in maize.
This study was carried out to obtain basic envirinmental information of the native area and growth characteristics at different age of Dendropanax morbifera LEV. In the southern Korea. The Dendropanax morbifera LEV. Was grown naturally at Wando, Cheju(Mt. Halla), Yecheon(Kermoon island),Sinan(Hong isand), Jindo(Mt. Chermchill) and Haenam(Mt. Taeroon), all between 30 to 450m above the sea level. The soil characterisitics of the native area had a pH of 5.3 to 5.4 with 8.4 to 9.8% content of organic matter and 18.2 to 21.3% soil moisture. Flowering starting in the middle of July begun when the trees were 6 years old. Six year old trees are 129 cm in stem height, 34 mm in stem diameter,15.4 in number of leaves with petioles 12.9 cm long. Xylem sap can be obtained from over 10 year old trees that grew well and have over 10 cm stems in diameter.
재배화(栽培化)가 되지 않아 자생품(自生品)을 채취(採取) 한약재(韓藥材)로 이용(利用) 되어 왔으며 수요량(需要量)도 높은 쇠무릎의 뿌리 수량(收量)을 높이기 위하여 지상부(地上部) 절단(切斷) 시험(試驗)을 수행(遂行)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수확기(收穫期) 경장(莖長)은 무절단(無切斷)이 103cm로 가장 길었고 20rm 절단(切斷) 및 개화직전절단(開花直前切斷)은 각각(各各) 86.82cm로 큰 차이가 없었으나 2회절단(回切斷)은 49cm로 찾았다. 2. 절단처리(切斷處理)에 따른 주당(株當) 꽃의 수(數바)는 20, 30, 2회(回) 절단(切斷) 등(等제) 절단량(切斷量)이 증가(增加) 할수록 감소(減少)가 컸으며 꽃대(화촉)의 길이도 짧아졌다. 3. 종자(種子) 결실량(結實量)은 무절단(無切斷), 20, 30cm 절단처리간(切斷處理間)에는 차이(差異)가 인정(認定)되지 않았으나 2회(回) 절단(切斷)은 결실량(結實量)이 가장 적었으며 개화직전절단(開花直前切斷)은 무절단(無切斷)에 비해 유의적(有意的)으로 적엇으며 이에 따른 종자등숙율(種子登熟率)도 비슷한 결과(結果)를 보였다. 4. 뿌리 직경(直徑)은 무절단(無切斷), 개화직전(開花直前) 절단(切斷)이 각각(各各) 7.1, 7.3츠였으나 그 외 절단처리(切斷處理)는 6.6~6.8 였고 주근장(主根長)은 개화직전(開花直前) 절단(切斷)이 가장 길었으며 개당(個當) 뿌리 무게도 개화직전(開花直前) 절단(切斷)이 21g으로서 무절단(無切斷)에 비해 3.5g이 높았다. 5. 건(乾)뿌리 수량(收量)은 무절단(無切斷) 253kg/10a에 거친 반면 개화직전(開花直前) 절단(切斷)은 285kg/10a로서 무절단(無切斷) 대비(對比) 13%가 증수되었다.