Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, many kinds of compounds have been evaluated for their nematicidal activity against PWN with different bioassay system according to researchers. Therefore, it was hard to directly compare their activity. To establish standard procedure for bioassay, nematicidal activity of abamectin was tested and compared in different conditions. The tested conditions are following: 1) nematode density: 100 and 1000 nematodes in 0.1 and 1 mL, 2) amount of reagent: 0.1, 1, 10, 100 uL of abamectin solution which same amount of abamectin was dissolved in. We will report the results of tested nematicidal activity according to conditions.

고추 비가림하우스에서 에어포그 시스템을 이용한 무인방제 효과를 평가하기 위해 본 시험을 수행하였다. 에어포그 시스템과 동력분무기를 이용한 관행방제의 약제살포 시간은 동일한 약량으로 살포하였을 때 처리 간에 차이가 없었으나, 에어포그 시스템으로 1/2약량을 살포하였을 때는 관행방제 보다 살포시간이 약 50% 줄었다. 약제부착 능력을 감수지로 조사하였을 때, 고추 생육초기와 중기에는 모든 처리에서 약제 부착이 우수 하였지만, 생육후기에는 관행방제의 약제 부착이 에어포그 시스템보다 균일하지 않았다. 처리별 총채벌레 살충률은 관행살포 90.0%, 에어포그 시스템 90.8%, 에어포그 시스템 1/2약량이 83.5% 로 유사한 방제효과를 나타내었다. 이러한 결과로 에어포그 시스템은 고추 비가림하우스 내에서 노동시간과 약제 살포량을 줄이면서 총채벌레를 효과적으로 방제할 수 있을 것으로 판단된다.

As concerns about the pursuit of well-being culture and interest in safe food has increased and demand for organic agricultural products are increasing. This study was conducted to investigate the occurrence pattern of diseases and pests and to select effective organic materials for control of diseases and pests in organic watermelon. The watermelon was damaged by diseases and pests such as Didymella bryoniae, Podosphaera xanthii, Aphis gossypii, Tetranychus urticae, thrips, Spodoptera exigua, and Spodoptera litura. Among them, powdery mildew and cotton aphid are major disease and pest in organic watermelon. The occurrence of powdery mildew and cotton aphid initiated in the middle of May in Chungju and Eumseong. The control efficacy for eleven organic materials against powdery mildew was evaluated. As the results, the symptoms of plant disease were effectively reduced by more than 85% in the treatments of materials such as sodium bicarbonate 80% and sulfur 80%. The treatment of TFM-6 (insect repellent 2%+paraffin oil 68%) and TFM-7(cinnamomum ext. 10%+derris ext. 20% + citranella oil 30%) showed by more than 80% in control efficiency against cotton aphid. Tstudy was supported by the research project of RDA.(Project No.: PJ0125302018)

Spotted wing drosophila, Drosophila suzukii, causes damage on several fruits include strawberry in Korea. D. suzukii lays egg inside fruit surface, and this behavior makes D. suzukii hard to detect, so many countries designated D. suzukii as a quarantine pest. Recently, Australian government demand methyl bromide fumigation on Korean export strawberry to prevent D. suzukii invasion. In this study, we tested methyl bromide to control D. suzukii, and also tested separate and concurrent treatments of methyl bromide to evaluate phytotoxicity on strawberry. When 40 g/m3 of methyl bromide was treated for 3 hours, all stages of D. suzukii were completely controlled. When treated separate and concurrent with cold temperature treatments of methyl bromide, there’s no specific phytotoxicity on strawberry. This result indicates that separate and concurrent treatments of methyl bromide do not cause phytotoxic effect on strawberry and methyl bromide is effective to control D. suzukii.

The effect of fumigation on the phosphine-susceptible and -resistant strains in the T. castaneum was evaluated using phosphine, ethyl formate, and combination with phosphine and ethyl formate (phosphine+ethyl formate). The Lethal Concentration Time (LCT)50 analysis of susceptible strains, late larva showed that phosphine (0.13 mg·h / L), ethyl formate (80.91 mg·h / L), and phosphine + ethyl formate (19.36 mg·h / L). The LCT50 of adult was 0.05 mg·h / L, 68.58 mg·h / L and 17.84 mg·h / L when treated with phosphine, ethyl formate, and phosphine + ethyl formate. The LCT50 of resistant strains, late larva was found to 33.32 mg·h / L of phosphine, 113.46 mg·h / L of ethyl formate and 129.85 mg·h / L of phosphine + ethyl formate, and the LCT50 of adult was 55.71 mg·h / L of phosphine, 85.39 mg·h / L, phosphine + ethyl formate 85.83 mg·h / L. The treatment of three fumigants (phosphine, ehtyl formate, and phosphine+ethyl formate) showed the possibility of controlling against T. castaneum of phosphine-susceptible and –resistant strains.

The Ryanodine Receptor in wild type Drosophila melanogaster has an amino acid substitution which is known to cause Chlorantraniliprole resistance in Pluteall xylostella. Even though we have reported that two Chlorantraniliprole resistant Drosophila strains have elevated total esterase activities, our report does not fully explain the significantly increased resistant ratios in two Chlorantraniliprole resistant Drosophila strains. Thus, we further analyzed alteration of reactive oxygen species and mitochondria activities in two Chlorantraniliprole resistant Drosophila strains. Our result suggested that Chlorantraniliprole resistance development in Drosophila requires alteration of various signal transduction pathways.

Methyl bromide (MB) has been still routinely used in quarantine fumigation on imported citrus, although there had been issuing chronic inhalation toxicity to fumigators and related workers as well as phytotoxic damages after fumigation. Ethyl formate (EF), is the only option to replace MB in terms of its safety for consumers (food additive and naturally occurred) and worker with higher threshold level limit (TLV = 100 ppm). Its application technology also provide cost effectiveness, good commercial practice in terms of application time (< 10 min) for 40 ft container. The replacement of MB with EF is recommended not to fumigate with hazardous and phasing-out MB on imported oranges.

The fumigation toxicity of carbonyl sulfide to T. castaneum as a storage grain pest was evaluated. Carbonyl sulfide (COS) is registered in Australia for microorganism present in soil, root and fertilizer. the fumigation activity of carbonyl sulfide was investigated in 12 L desiccator for 24 h exposure to eggs, larvae, pupae, adults of T. castaneum. Eggs and pupae were showed 87.3% and 95.6% mortality for 25 mg/L of COS, respectively. Larvae and adults were investigated with 80.0% and 100.0% mortality at 15 mg/L treatment, respectively. Therefore, the eggs of T. castaneum showed the highest tolerance to COS.

The fumigation activity of phosphine (PH3) to T. castaneum as a storage grain pest was evaluated. The lethal concentration time (LCT) value of each developmental stage (egg, early larva, late larva, pupa and adult) of T. castaneum was analyzed in 12 L desiccator. At the T. castaneum larva stages, exposure for 4 h showed low LCT value, especially in early stage larvae (LCT99 = 0.32 mg·h/L) which is very high susceptibility to PH3. However, T. castaneum eggs were observed very high tolerance to PH3 at LCT99 77.47 mg·h/L. Therefore, the fumigant activity of PH3 against T. castaneum can be found to be significantly different depending on developmental stage.

친환경 벼 재배지역을 중심으로 점차 피해가 확대되는 먹노린재(Scotinophara lurida) 방제기술 개발을 위하여 전남지역에서 발생생태 및 온도의존 발육모형을 조사하였다. 2019년 봄 전남 6개지역에서 먹노린재의 월동후 생존율을 조사한 결과 총 153마리중 104마리가 생존하여 67.6%의 생존율을 보였다. 먹노린재는 벼 이앙 후 6월 중하순부터 월동성충이 발생하기 시작하여 7월 상중순에 산란하고 8월 중하순에 1세대 성충이 발생한다. 온도 의존 발육모형을 구하기 위하여 18, 21, 24, 27, 30℃ 14L:10D 조건의 항온기에서 온도별, 발육단계별 발육기간을 조사하였다. 18, 21, 24, 27, 30℃에서 알에서 성충이 될 때까지 소요기간은 각각 119.8, 73.1, 53.5, 39.4, 82.0일로 27℃에서 가장 발육이 잘 되었다. Excell 프로그램을 이용하여 온도와 발육속도와의 관계를 2차 다항식으로 분석하여 회귀곡선을 얻었고 이를 근거로 발육단계별 발육영점온도와 유효적산온도를 산출하였다. 먹노린재의 알에서부터 5령 약충까지의 발육영점온도는 17.9℃, 유효적산온도는 380.2일도였다.

수노랑나비(Chitoria ulupi (Doherty, 1889))는 한반도 전역에 국지적으로 분포하는 종으로, 국외의 경우 인도, 미얀마, 라오스, 중국, 대만 등에 서식하는 것으로 알려져 있다. 이 종에 대한 생활사는 김과 서(2012)에 의해 확인된 유생기와 번데기의 형태 등이 일부 보고 된 것 이외에는 알려진 바가 없다. 본 연구에서는 한국산 수노랑나비의 생태적 습성(성충 산란행동, 점유행동, 짝짓기, 먹이습성 등)과 미성숙단계 (알, 유충, 번데기)의 형태적 특징 등 생활사 전반에 대하여 보고 하고자 한다.

Honey bees are affected by a variety of factors, so they have to be thoroughly managed according to their lifestyle. The activity of the honey bee foragers represent an important parameter of the hive state. Here, the real-time and automatic monitoring system using dual infrared sensors was applied for counting the foraging activity of honey bees based on ICT. According to this study, this system is very accurate with a relative error of 3.98% / 4.43% compared to manual counting through video analysis. This system showed the scalability of the system through the internal and external temperature sensors connected through the main board and BLE module. Furthermore, the data measured through this system for one month were analyzed, the monthly average foraging activity and the number of lost foragers were measured (1.88% of outgoing bees), and at the same time, the foraging patterns according to the changes of temperature and time were analyzed. This study suggests that the development of apicultural, scientific and educational materials with more powerful real-time monitoring tools through expansion of a complex monitoring system and big data accumulation.

경북 영양지역 고추 비가림하우스 재배지에서 2016년부터 2018년까지 주요 해충의 발생양상을 조사하였다. 발생조사는 영양읍, 청기면, 일월면을 중심으로 임의 10개 포장을 선정하여 4월부터 10월까지 실시하였다. 총채벌레류는 5월 중순, 6월 중순, 7월 상순∼중순, 9월 중순∼9월 하순에 걸쳐 총 4회의 발생최성기를 보였고 꽃노랑총채벌레가 84.8%로 우점하였다. 총채벌레 피해과율은 조사연도에 따라 0.8~7.3%로 조사되었다. 담배나방 유충에 의한 과실피해 는 7월 상순, 8월 중순, 9월 중순~9월 하순에 많이 발생하였다. 진딧물류는 5월 중순～5월 하순, 7월 중순∼8월 하순에 걸쳐 연 2회의 발생최성기를 보였다. 가루이류는 8월 중순～8월 하순, 9월 하순～10월 상순에 걸쳐 연 2회의 발생최성기를 나타냈으며, 담배가루이가 98.1%, 온실가루이가 1.9%로 담배가루이가 우점종인 것으로 확인되었다.

Riptortus pedestris is a polyphagous species and an economically important insect pest of soybean. We investigated the development time, survivorship, longevity and fecundity of R. pedestris at five different temperatures. Life table analysis is the prime tool in population dynamics and pest management. We applied the computer programs – TWOSEX-MSChart and TIMING-MSChart - to analyze our data. We obtained the population parameters – adult preoviposition period (APOP), total preoviposition period (TPOP), oviposition days, eggs per reproductive female, first age of survival rate <50%, proportion of male and female individuals and life table parameters (net reproductive rate, intrinsic rate of increase, finite rate of increase, and mean generation time) - and population projection using two computer programs.

The study conducted to understand the annual phenology of hemipteran insect pests including Halyomorpha halys, Nezara antennata, Plautia stali, and Riptortus pedestris in Jeju, Korea, 2017 and 2018. We investigated the population density of them at three locations for two years. The commercial and fish trap were used for monitoring. We compare the seasonal occurrence among species and sampling locations. The repeated measured ANOVA was used to explain the difference of seasonal occurrence among locations and sampling time. There was difference the first adult catch and the peak occurrence of species among sampling sites.

A tick survey was conducted to monitor ticks using tick traps attached dry ice and a flagging method at each four sites in Busan, Ulju, and Geoje areas of Gyeongnam Province from April to November, 2018. Haemaphysalis longicornis was the most frequently collected, representing 99.8% in Ulju. A total of 27,247 ticks were collected as H. longicornis (Trap Index; TI 283.2), H. flava (TI 0.3), and Ix. nipponensis (TI 0.3). In the collection environment, a total number of 15,672, 5,798, 4,876, and 901 ticks were collected from glassland, tump, tomb, and mountain path, respectively. On the other hand, H. longicornis and Ix. nipponensis were collected with flaggings at the four sites in Ulju, Busan, and Geoje in May and June, 2018. A total of 1,517 ticks collected H. longicornis (Flagging Index; FI 9.3) and Ix. nipponensis (FI 0.1) in Ulju. Haemaphysalis longicornis were collected 2,183 (FI 13.6) and 1,596 (FI 10.0) in Geoje and Busan, respectively. In the results of the isolation of Severe Fever with Thrombocytopenia Syndrome (SFTS) from the ticks, no pathogens detected from RNA of 557 pools of the ticks using a Polymerase Chain Reaction method in 2018.

본 연구의 조사지역인 갑천은 충청남도 금산군·논산시와 대전광역시를 북류하고 금강으로 흘러드는 도심하천이다. 광대한 갈대 군락과 초원, 버드나무 등 다양한 식생이 분포하며 크고 작은 자연 소택지, 늪, 웅덩이 등의 형태로 자연환경이 잘 보존되어 도시생태계의 주요 기능을 담당하고 있다. 본 연구에서 갑천 일대의 육상곤충류를 대상으로 4월 11~13일, 6월 11~12일, 8월 20~22일 조사를 수행하였다. 조사방법은 직접찾기와 쓸어잡기로, 총 9개 지점으로 나누어 군집 분석하였다. 조사 결과 공단구역인 Site3의 다양성지수가 0.16으로 매우 낮았으며 도심구역인 Site4와 Site7이 각각 다양성지수 2.92와 2.41로 타구역에 비해 낮았다. 반면 산림구역인 Site1, Site8 그리고 Site9는 모두 다양성지수가 3이상으로 높은 편에 속했다.

본 조사지인 돌리네 습지보호지역은 경북 문경시 산북면 굴봉산 정상부에 위치하였으며, 물이 고이기 힘든 돌리네(doline) 지형에 형성된 희귀 습지이다. 뿐만 아니라 멸종위기종과 법적보호종이 분포하는 등 생물다양성 또한 매우 풍부한 것으로 평가되고 있다. 그러나 습지보호지역 주변으로 과수원 등 경작지가 형성되어 있어, 관리 및 보전을 위한 조사 및 대책이 필요하다. 따라서 본 조사에서는 습지보호지역의 체계적인 관리를 위하여 돌리네 습지의 곤충상을 조사하였다. 조사 방법에는 곤충의 다양한 습성과 서식환경을 고려하여, Searching, Sweeping, Pitfall trap, Bucket trap으로 총 4가지의 채집방법으로 조사를 실시하였다. 그 결과 총 11목 82과 188속 237종이 확인되었으며, 이중 딱정벌레목이 가장 많이 출현하였다. 특이사항으로 환경부지정종 51종(중복포함)이 조사되었으며, 그중 멸종위 기종 1종이 확인되었다. 본 연구를 통해 돌리네 습지보호지역의 곤충상을 파악하고 관리 및 보전에 대한 기초자료를 제공하고자 한다.

The leafhopper family Cicadellidae (Hemiptera: Auchenorrhyncha) is plant feeders, with mouthparts adapted for sucking. Almost of the them cause injuries of destruction to plants, including fruit trees and grain crops, and can be vectors of plant diseases. Therefor, Cicadellidae is one of the most important pests in the world. However, their identifications are very difficult because of small size and morphological similarities, and their exact biological information such as host plants, life cycle and distribution are not clear for most species. So, we provide and integrated biological database that present accurate diagnostic methods and ecological infromation.

The Celyphidae, commonly known as beetle flies or beetle-backed flies, are a family of Diptera. The scutellum is enlarged and forming a protective shell over the abdomen, giving them a beetle-like appearance. Species in the family are mostly found in tropical region, especially in South East Asia. Records of North East Asia are rare, in China, some species are found in Southern region. In Japan, few species found in Okinawa region, but only one record of Celyphidae near in mainland area. In this research we firstly record specimen of genus Spaniocelyphus sp., from Korea stored in National Science Museum Korea and Kyongpook National University with comparison of specimen from Tsushima island, Nagasaki prefecture stored in Osaka Natural History Museum.