A packed bed of volcanic rock was used as deodorizing material to remove hydrogen sulfide(H2S) from air in a laboratory-scale column, and was inoculated with Thiobacillus sp. as H2S oxidizer. The effects of volcanic rock particle size distribution on system pressure drop were examined. Various tests have been conducted to evaluate the effect of H2S inlet concentration and EBCT(Empty Bed Contact Time) on H2S elimination. The pressure drop for particles of size range from 5.6 to 10 ㎜ was 14 ㎜H2O/m at a representative gas velocity of 0.25m/s. Biofilter using scoria and Thiobacillus sp. could get the stable removal efficiencies more than 99.9% under H2S inlet concentrations in the range from 30 to 1,100ppm at a constant gas flow rate of 15.2 ℓ/min. H2S removal efficiencies greater than 99% were observed as long as EBCT was longer than 8sec at the 250ppm of H2S inlet concentration. When EBCT was reduced to 5.5 sec, H2S removal efficiency decreased by about 12 percent. The maximum H2S elimination capacity was determined to be 269g-H2S/㎥·hr.