Methyl jasmonate(MeJA) treatment promotes the plant secondary, especially triterpene saponin which is main compounds in Platycodon grandiflorum. We were performed 5,760 expressed sequence tags from MeJA treated hairy root. Total number of ESTs are 2,536 (811 contigs and 1,725 singletons) among them 2,299 are pass the annotation. The most abundant transcript were encoding fatty acid desaturase (FAD) and lots of secondary metabolite transcript were observed. Four MVA pathway genes (HMGS, HMGR, MK, MDD) and one MEP pathway gene (IDI) are identified. The unigene were classified into three major functional categories by standard GO (Gene Ontology) - cellular component, molecular function and biological process. In this study we were cloned four genes from ESTs : HMGS (1401bp ORF), MK (1167bp ORF), MDD (1254bp ORF), IDI (909bp ORF)

The composition and distribution of seed storage proteins are important factors for eating quality such as grain flavor and quality in rice (OryzasativaL.) Rice protein disulfide isomerase (OsPDI) and binding protein (OsBIP) regulate synthesis, stability and sorting of storage proteins. We thus have tried to develop a marker protein for selection of rice cultivars which have different eating quality. Immunoblot analysis revealed that protein levels of OsPDI and OsBIP have no direct correlation with eating quality, suggesting that they may indirectly participate in control of eating quality through their-interacting partners or other regulatory mechanism.

The aim of this investigation was to examine the influence of extrusion on dietary fiber profile and the contentof bioactive compounds, rutin and quercetin in young sprout, whole seed, and matured stem of Tartary buckwheat.WSI(water soluble index) is increased by a function of both screw profile and process temperature, compared to control indifferent parts of Buckwheat. Also, WSI of ME is increased more than 5.2 times in grain, compared to that of control. Theeffect of precooking by extrusion on the dietary fiber profile of buckwheat flour was evaluated. Precooking by extrusion sig-nificantly increased SDF in flour, although in most cases extrusion decrease in TDF a little. The thermo-mechanical treat-ment undergone by the buckwheat flour during extrusion led to redistribute part IDF fraction to SDF, leading to an increasein the latter. The content of rutin was increased about two fold in extruded flour of sprout, compared to in control. Thisincrease maybe why these compounds are released from cell wall by high shear processing under high temperature.

Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a F2 population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the F2 population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the F2 population will be used for artificial selection and improvement of giant embryonic varieties.

"Seoanl" is a new japonica rice variety developed from a cross between Namyang9, a line of lodging tolerance and adaptable to reclaimed saline area, and Gyehwa7, a line of good-quality, adaptable to reclaimed saline area and high yield by the rice breeding team of National Institute of Crop Science, RDA, in 2005. This variety has about 131 days of growth duration from transplanting to harvesting in central plain area, and mid-western coastal area in Korea. It is about 73 cm in culm length and tolerance to lodging. It has also semi-erect pubescent leaf blade and slightly tough culm with good canopy architecture. The number of panicles per hill of Seoanl is slightly more than that of Hwaseongbyeo. Seoanl was susceptible to major diseases, blast, bacterial blight and viruses, and insect, brown plant hopper. This variety has lower amylose content of 18.8% and better palatability of cooked rice than those of Hwaseongbyeo. The milled rice yield performance of this variety is about 5.60MT/ha in local adaptability test for three years. Seoanl would be adaptable to central plain area and mid-western coastal area of Korea.

"Samkwangbyeo" is a new japonica rice variety developed and registered by the rice breeding team of National Institute of Crop Science, RDA in 2003, which was derived from a cross between good quality, high yielding line Suweon361 and a disease resistant line Milyangl01. This variety has about 129 days duration from seeding to heading in central lowland and mid-mountainous areas. It is about 87 cm in culm length and moderately tolerant to lodging. It has also erect pubescent leaf blade and slightly tough culm with good canopy architecture. Samkwangbyeo has the same number of tillers per hill and spikelets per panicle as those of Ilpumbyeo. It shows less sensitivity to premature heading by lately transplanting of 50-day old seedlings. It is slightly less tolerant to cold stress in terms of both heading delay and spikelet fertility than those of Ilpumbyeo. It shows delayed leaf senescence and considerable tolerance to viviparous germination at ripening stage. It shows moderate resistance to leaf blast, bacterial blight, and stripe virus, but susceptibility to insect pests. The milled rice of Samkwangbyeo exhibits translucent, relatively clear non-glutinous endosperm and medium short grain. It has similar amylose content of 18.3% and similar gelatinization temperature, and good palatability of cooked rice compared to Ilpumbyeo. The milled rice yield of Samkwangbyeo is about 5.69 MT/ha at ordinary culture in local adaptability test for three years. Samkwangbyeo would be highly adaptable to central lowland and mid-southern mountainous areas of Korea.