The influences of ethylene inhibitors (AgNO3 and silver thiosulfate) and cytokinins (BAP and TDZ) on shoot regeneration from cotyledon and hypocotyl explants of B. napus cv. Youngsan were investigated. The presence of 50 μM Silver thiosulfate (STS) in shoot regeneration medium formed shoots at 60-68% after 3-4 weeks of culture, which was enhanced by 2-fold compared to that of Silver nitrate (AgNO3). Moreover, cotyledon explants were more regenerative than hypocotyls; shoots from cotyledon explants began to occur 4-5 days earlier than that of hypocotyl explants. TDZ at a concentration of 8-10 μM was effective for shoot regeneration, compared with BAP. Consequently, the optimal shoot regeneration response was observed in medium supplemented with 50 μM STS + 8 μM TDZ. In transmission electron microscopy (TEM) analysis, higher density of silver nanoparticles was shown to be accumulated widely inside the cell wall and plasmodesmata of regenerating leaf cultured in medium supplemented with AgNO3. By contrast, in the cell cultured in medium with STS, fine-grained deposits were partly observed in the surroundings of the cell wall.

The high quality of gene set is necessary to study the functional research of genes. Although perilla is cultivated as an oil crop and as a vegetable crop in Asian countries such as Korea, Japan, northeast China and Nepal, the reference genome is absent. To assembly perilla gene set, we sequenced the various tissues of perilla (Perilla citriodora) RNA-seq with Illumina HiSeq platform, generating 548,549,314 short reads. When de novo transcriptome assembly was performed with five samples, 86,396 and 38,413 transcripts were assembled as total and representative transcripts, respectively. Using 1,917,424 proteins at Phytozome ver. 9.1, we annotated the perilla assembled transcripts, and 66,139(76.55%) and 24,030(62.55%) transcripts showed the similarity with known plant proteins (E-value < 1e-10) as total and representative transcripts, respectively. Among the diverse molecular functions, we were interested in the regulatory components, such as transcription factor and transcription regulator. Using this data, we identified 499 transcripts annotated the putative transcription factor differentially expressed transcripts. 165 putative transcription factors were significantly expressed in perilla flower and 121 putative transcription factors in both leaf and flower. This study provides the perilla reference gene set and the understanding of the molecular regulation of transcription factor dependent on the tissue.

A new standard rose cultivar 'White Christmas' was bred from the cross between red standard cultivar 'Red Queen' and pink standard cultivar 'Vivaldi' at the National Horticulture Research Institute. The cross was made in 2001 and `White Christmas' was finally selected in 2006 after investigating characteristics for three years from 2003 to 2006. 'White Christmas', a white standard cultivar grows vigorously and has good flower shape. The major characteristics of this cultivar are 123.8 stems/m2/year in yield, 82.3 cm in length of cut flower, 11.0 cm in flower diameter, 60.1 in petal number, and 12.2 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'Tineke'.

A new standard rose cultivar 'Magic Pink' was bred from the cross between light yellow standard cultivar 'Medeo' and pink standard cultivar 'Veronica' at the National Horticulture Research Institute. The cross was made in 2001 and 'Magic Pink' was finally selected in 2006 after investigating characteristics for three years from 2003 to 2006. 'Magic Pink', a pink standard cultivar grows vigorously and has fashionable flower color. The major characteristics of this cultivar are 184.8 stems/m2/year in yield, 63.0cm in length of cut flower, 7.2 cm in flower diameter, 28.5 in petal number, and 10.4 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'Noblesse'.

spray cultivar “Yellow Mimi” at the National Horticulture Research Institute. The cros was made in 199 and the “Suny Lady”was finaly selected in 2003 after investigating characteristics for thre years from 2001 to 2003. “Sunny Lady”, a yellow spray2/year

orange-red standard cultivar “Baroness” at the National Horticulture Research Institute. It was crossed in 20 and finally selectedin 2004 after the investigation of the characteristics for three years from 202 to 204. “Pearl Red”, red standard flower, has